BP303T. PHARMACEUTICAL MICROBIOLOGY Theory

Pharmaceutical Microbiology Theory syllabus

Unit I Introduction

History of microbiology, (MCQ Video Link: Click here)

Branches of microbiology,

Scope of microbiology,

Introduction to Prokaryotes and Eukaryotes

Study of ultra-structure of bacteria

Morphological classification of bacteria,

Nutritional requirements,

Raw materials used for culture media (MCQ Video Link: Click here) (Video link: Click here)

Physical parameters for growth, Growth curve (MCQ Video Link: Click here)

Isolation of pure cultures (MCQ Video Link: Click here)

Preservation methods for bacteria

Cultivation of anaerobes,

Quantitative measurement of bacterial growth (total & viable count).

Microscopy: Study of different types of phase contrast microscopy, darkfield microscopy, and electron microscopy.

Unit II

Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and

Biochemical tests (IMViC).

Study of principle, procedure, merits, demerits, and applications of physical, chemical gaseous, radiation, and mechanical method of sterilization.
(MCQ Video Link: Sterilisation Part I, Sterilisation Part II)

Evaluation of the efficiency of sterilization methods.

Equipment employed in large-scale sterilization.

Sterility indicators.

Unit III


Study of morphology, classification, reproduction/replication, and cultivation of Fungi and Viruses.

Classification and mode of action of disinfectants

Factors influencing disinfection, antiseptics, and their evaluation.

For bacteriostatic and bactericidal actions: Evaluation of bactericidal & Bacteriostatic.

Sterility testing of products (solids, liquids, ophthalmic, and other sterile products) according to IP, BP, and USP.

Unit IV

Designing of aseptic area, laminar flow equipment;

study of different sources of contamination in an aseptic area and methods of prevention, clean area classification.

Principles and methods of the different microbiological assay.

Methods for standardization of antibiotics, vitamins, and amino acids.

Assessment of a new antibiotic.

Unit V

Types of spoilage,

Factors affecting the microbial spoilage of pharmaceutical products, sources, and

Types of microbial contaminants,

Assessment of microbial contamination and spoilage.

Preservation of pharmaceutical products using antimicrobial agents,

Evaluation of microbial stability of formulations.

Growth of animal cells in culture, the general procedure for cell culture,

Primary, established and transformed cell cultures.

Application of cell cultures in pharmaceutical industry and research.

  1. W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific
    publications, Oxford London.
  2. Prescott and Dunn., Industrial Microbiology, 4th edition, CBS Publishers & Distributors,
    Delhi.
  3. Pelczar, Chan Kreig, Microbiology, Tata McGraw Hill edn.
  4. Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
  5. Rose: Industrial Microbiology.
  6. Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
  7. Cooper and Gunn’s: Tutorial Pharmacy, CBS Publisher and Distribution.
  8. Peppler: Microbial Technology.
  9. I.P., B.P., U.S.P.- latest editions.
  10. Ananthnarayan : Text Book of Microbiology, Orient-Longman, Chennai
  11. Edward: Fundamentals of Microbiology.
  12. N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
  13. Bergeys manual of systematic bacteriology, Williams and Wilkins- A Waverly company

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