Cell culture, Preservatives, and microbiological spoilage MCQ with answers
______is used to evaluate the efficacy of preservatives.
Preservative characteristic test
Preservative challenge test
Preservative activity test
Protection challenge test
Preservative characteristic test
Preservative challenge test
Preservative activity test
Protection challenge test
Answer: Preservative challenge test
LAL stands for
Limulus Amoebocyte Lysate test
Limulus Amoeba Lysate test
Litmus Amoebocyte Lysate test
Limulus Amoebocyte Lysergic acid test
Limulus Amoebocyte Lysate test
Limulus Amoeba Lysate test
Litmus Amoebocyte Lysate test
Limulus Amoebocyte Lysergic acid test
Answer: Limulus Amoebocyte Lysate test
Water for injection should be held at _______ degree celsius and above after distillation to prevent the growth of microorganisms.
100
80
70
60
100
80
70
60
Answer: 80
The microbial limit test (MLT) is performed to assess how many and which of certain _________are present in non-sterile pharmaceuticals.
Microorganisms
Viable microorganisms
Preservatives
Antimicrobial
Microorganisms
Viable microorganisms
Preservatives
Antimicrobial
Answer: Viable microorganisms
LAL test is used for the detection of —–
bacterial endotoxin
Bacterial exotoxins
Viral toxins
Bacterial and viral exotoxins
bacterial endotoxin
Bacterial exotoxins
Viral toxins
Bacterial and viral exotoxins
Answer: bacterial endotoxin
Raw materials are more prone to microbial contamination except from _____
Plant origin
Synthetic origin
Animal origin
Vegetable origin
Plant origin
Synthetic origin
Animal origin
Vegetable origin
Answer: Synthetic origin
To reduce microbial growth all walls, floors and ceilings should be _________.
Coated with stainless steel
Should be painted with antibacterial paint
removable and washable
Smooth and washable
Coated with stainless steel
Should be painted with antibacterial paint
removable and washable
Smooth and washable
Answer: Smooth and washable
Following are the factors affecting microbial growth except for___
Nutrients
pH
temperature
Adsorbent
Nutrients
pH
temperature
Adsorbent
Answer: Adsorbent
E-coli shows_____.
Indole and methyl red test +ve
Indole and methyl red test -ve
Vogus pouasker and citrate +ve
Indole and citrate test –ve
Indole and methyl red test +ve
Indole and methyl red test -ve
Vogus pouasker and citrate +ve
Indole and citrate test –ve
Answer: Indole and methyl red test +ve
The optimum temperature for storage of cell lines is ______
below -130°C
at 24°C
at 8°C
at 0°C
below -130°C
at 24°C
at 8°C
at 0°C
Answer: below -130°C
_________is the dissociation of cells from a parental animal or plant tissue through enzymatic or mechanical measures and maintaining the growth of cells.
Established cell culture
Primary cell culture
Transformed cell cultures
Secondary cell culture
Established cell culture
Primary cell culture
Transformed cell cultures
Secondary cell culture
Answer: Primary cell culture
Established cell culture grows ______ than primary cell culture.
Slower
Faster
Similar
Different
Slower
Faster
Similar
Different
Answer: Faster
Assessment of microbial contamination and spoilage can be done by________
Sterility test
Preservative challenge test
IMViC test
Media sterilization test
Sterility test
Preservative challenge test
IMViC test
Media sterilization test
Answer: Sterility test
As per indian Pharmacopoeia microbial limit test, total viable count of E coli in lactose should _________,
<100/gm
<1000/gm
<500/gm
<50/gm
<100/gm
<1000/gm
<500/gm
<50/gm
Answer: <100/gm
The total viable aerobic microbial count present in the pharmaceutical product can be determined by________.
Membrane filtration
Cup plate method
Electric counter
Dry weight method
Membrane filtration
Cup plate method
Electric counter
Dry weight method
Answer: Membrane filtration
In the detection test of salmonella, it is subcultured on ____________media.
Soyabean casein digest agar
MacConkey agar
Xylose lysine deoxycholate agar
Sabouraud dextrose agar
Soyabean casein digest agar
MacConkey agar
Xylose lysine deoxycholate agar
Sabouraud dextrose agar
Answer: Xylose lysine deoxycholate agar
Animal cell culture technology is being applied mainly in the production of
Vaccine
Insulin
Interferons
Edible proteins
Vaccine
Insulin
Interferons
Edible proteins
Answer: Vaccine
The presence of some surfactants, suspending agents and proteins can ________of microorganisms to preservatives.
Increase the resistance
Decrease the resistance
Decrease the sensitivity
Enhance growth
Increase the resistance
Decrease the resistance
Decrease the sensitivity
Enhance growth
Answer: Increase the resistance
A finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes_______.
Transformed cell line
Continuous cell line
Established cell culture
Primary cell line
Transformed cell line
Continuous cell line
Established cell culture
Primary cell line
Answer: Continuous cell line
After more than ____ subculturing, there is an alteration in cell division characteristics and cell culture becomes transformed cell culture.
50
70
100
80
50
70
100
80
Answer: 80
Microbiology MCQ with answers:
- Unit I MCQ Introduction to Microbiology, Microscopy, Bacteria
- Unit 2 MCQ Staining techniques, Biochemical tests, and methods of sterilization
- Unit 3 MCQ Fungi and Viruses, Antiseptics and disinfectants
- Unit 4 MCQ Microbiological assay, test, and aseptic area
- Unit 5 MCQ Cell culture, Preservatives, and microbiological spoilage
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