February 23, 2024

Cell culture, Preservatives, and microbiological spoilage MCQ with answers

Cell culture, Preservatives, and microbiological spoilage MCQ with answers

______is used to evaluate the efficacy of preservatives.
Preservative characteristic test
Preservative challenge test
Preservative activity test
Protection challenge test

Answer: Preservative challenge test

LAL stands for
Limulus Amoebocyte Lysate test
Limulus Amoeba Lysate test
Litmus Amoebocyte Lysate test
Limulus Amoebocyte Lysergic acid test

Answer: Limulus Amoebocyte Lysate test

Water for injection should be held at _______ degree celsius and above after distillation to prevent the growth of microorganisms.
100
80
70
60

Answer: 80

The microbial limit test (MLT) is performed to assess how many and which of certain _________are present in non-sterile pharmaceuticals.
Microorganisms
Viable microorganisms
Preservatives
Antimicrobial

Answer: Viable microorganisms

LAL test is used for the detection of —–
bacterial endotoxin
Bacterial exotoxins
Viral toxins
Bacterial and viral exotoxins

Answer: bacterial endotoxin

Raw materials are more prone to microbial contamination except from _____
Plant origin
Synthetic origin
Animal origin
Vegetable origin

Answer: Synthetic origin

To reduce microbial growth all walls, floors and ceilings should be _________.
Coated with stainless steel
Should be painted with antibacterial paint
removable and washable
Smooth and washable

Answer: Smooth and washable

Following are the factors affecting microbial growth except for___
Nutrients
pH 
temperature
Adsorbent

Answer: Adsorbent

E-coli shows_____.
Indole and methyl red test +ve
Indole and methyl red test -ve
Vogus pouasker and citrate +ve
Indole and citrate test –ve

Answer: Indole and methyl red test +ve

The optimum temperature for storage of cell lines is ______
below -130°C
at 24°C
at 8°C
at 0°C

Answer: below -130°C

_________is the dissociation of cells from a parental animal or plant tissue through enzymatic or mechanical measures and maintaining the growth of cells.
Established cell culture
Primary cell culture
Transformed cell cultures
Secondary cell culture 

Answer: Primary cell culture

Established cell culture grows ______ than primary cell culture.
Slower 
Faster
Similar 
Different 

Answer: Faster

Assessment of microbial contamination and spoilage can be done by________
Sterility test
Preservative challenge test
IMViC test
Media sterilization test 

Answer: Sterility test

As per indian Pharmacopoeia microbial limit test, total viable count of E coli in lactose should  _________,
<100/gm
<1000/gm
<500/gm
<50/gm

Answer: <100/gm

The total viable aerobic microbial count present in the pharmaceutical product can be determined by________.
Membrane filtration
Cup plate method
Electric counter 
Dry weight method

Answer: Membrane filtration

In the detection test of salmonella, it is subcultured  on ____________media.
Soyabean casein digest agar 
MacConkey agar
Xylose lysine deoxycholate agar
Sabouraud dextrose agar

Answer: Xylose lysine deoxycholate agar

Animal cell culture technology is being applied mainly in the production of 
Vaccine
Insulin
Interferons 
Edible proteins

Answer: Vaccine

The presence of some surfactants, suspending agents and proteins can ________of microorganisms to preservatives.
Increase the resistance
Decrease the resistance 
Decrease the sensitivity 
Enhance growth

Answer: Increase the resistance

A finite cell line undergoes transformation and acquires the ability to divide indefinitely, it becomes_______.
Transformed cell line
Continuous cell line
Established cell culture
Primary cell line

Answer: Continuous cell line

After more than ____ subculturing, there is an alteration in cell division characteristics and cell culture becomes transformed cell culture.
50
70
100
80

Answer: 80


Microbiology MCQ with answers:


Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem IIIS Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I TheoryBP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology TheoBP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II PracticalBP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

MCQ:

BP302T Physical Pharmaceutics I Theory MCQ

BP304T Pharmaceutical Engineering Theory MCQ

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