Staining techniques, Biochemical tests, and methods of sterilization MCQ
The SIM broth used in the Indole Product test contains
Tyrosine
Tryptophan
Cysteine
Arginine
Tyrosine
Tryptophan
Cysteine
Arginine
Answer: Tryptophan
Mycobacterial cell wall contains a waxy substance composed of
Picric acid
Mycolic acid
Myristic acid
Carbonic acid
Picric acid
Mycolic acid
Myristic acid
Carbonic acid
Answer: Mycolic acid
Crystal violet stains the
Bacterial cytoplasm
Bacterial cell wall
Bacterial nucleus
Bacterial spores
Bacterial cytoplasm
Bacterial cell wall
Bacterial nucleus
Bacterial spores
Answer: Bacterial cell wall
Gram staining was developed by
German bacteriologist
Danish bacteriologist
Russian bacteriologist
American bacteriologist
German bacteriologist
Danish bacteriologist
Russian bacteriologist
American bacteriologist
Answer: Danish bacteriologist
The smear developed in the Acid-fast staining is observed under oil immersion objective lens with a magnification of …………………
10X
100X
1000X
40X
10X
100X
1000X
40X
Answer: 100X
Counter stain used in the Acid fast staining technique is
Methylene blue
Malachite green
Crystal violet
Safranin
Methylene blue
Malachite green
Crystal violet
Safranin
Answer: Malachite green
Simple staining technique can be used to determine
Reproductive cycle of cells
Presence of Peptidoglycan
Arrangement of bacterial cells only
Different types of bacterial cells
Reproductive cycle of cells
Presence of Peptidoglycan
Arrangement of bacterial cells only
Different types of bacterial cells
Answer: Arrangement of bacterial cells only
In the Gram staining technique, the counter stain safranin colours the bacterial cells
Red
Pink
Blue
Green
Red
Pink
Blue
Green
Answer: Pink
………..is used as a dye in simple staining
Gram safranin
Fuchsin stain
Acridine orange
Crystal violet
Gram safranin
Fuchsin stain
Acridine orange
Crystal violet
Answer: Crystal violet
Bacterial cells are stained in _____
Negative staining
Simple staining
Cell wall staining
Capsule staining
Negative staining
Simple staining
Cell wall staining
Capsule staining
Answer: Simple staining
Liquid Parenteral preparations can be sterilized through?
Chemical sterilization
Membrane filtration
Desiccation
Red heat
Chemical sterilization
Membrane filtration
Desiccation
Red heat
Answer: Membrane filtration
Which of the following method is used for sterilization of Nichrome wire loop?
Red heat
Membrane filtration
Chemical method
Radiation sterilization
Red heat
Membrane filtration
Chemical method
Radiation sterilization
Answer: Red heat
Sterilization is?
Process of growing microbes in the laboratory
Process for the preparation of antibiotics
Killing or removing all the microorganisms
Estimation of potency
Process of growing microbes in the laboratory
Process for the preparation of antibiotics
Killing or removing all the microorganisms
Estimation of potency
Answer: Killing or removing all the microorganisms
Which of the following process does not kill bacterial endospores?
Hot air sterilization
Pasteurization
Autoclave
Incineration
Hot air sterilization
Pasteurization
Autoclave
Incineration
Answer: Pasteurization
The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the
thermal death time
thermal death point
decimal reduction time
thermal death temperature
thermal death time
thermal death point
decimal reduction time
thermal death temperature
Answer: thermal death point
Which of the following is bactericidal?
Membrane filtration
Freeze-drying
Ionizing radiation
Incubation
Membrane filtration
Freeze-drying
Ionizing radiation
Incubation
Answer: Ionizing radiation
____ is used to prevent infection by killing or inhibiting pathogen growth on animal tissues.
sterilant
disinfectant
adsorbent
antiseptic
sterilant
disinfectant
adsorbent
antiseptic
Answer: antiseptic
Incineration is method of _______ sterilisation
Moist heat
Dry heat
Filtration
Radiation
Moist heat
Dry heat
Filtration
Radiation
Answer: Dry heat
_______ is used to sterilise metallic objects
Incineration
Red heat
Flaming
Sunlight
Incineration
Red heat
Flaming
Sunlight
Answer: Red heat
Indole Production test identifies the following gram negative intestinal bacteria
Escherichia Coli
Streptocococcus
Bacillus antracis
Clostridium
Escherichia Coli
Streptocococcus
Bacillus antracis
Clostridium
Answer: Escherichia Coli
Reagents used in the Voges-Proskauer test are
Fehling solution 1 and 2
Kovac’s reagent
Millon’s reagent
A and B Barritt’s reagent
Fehling solution 1 and 2
Kovac’s reagent
Millon’s reagent
A and B Barritt’s reagent
Answer: A and B Barritt’s reagent
Primary stain used in Acid fast staining is
Crystal violet
Carbol Fuschin
Gram safranin
Malachite green
Crystal violet
Carbol Fuschin
Gram safranin
Malachite green
Answer: Carbol Fuschin
Simple staining uses dyes which are ………in nature for staining most of the bacteria
Acidic
Basic
Neutral
Acidic as well as Basic
Acidic
Basic
Neutral
Acidic as well as Basic
Answer: Basic
………..is used as decolorizer in the Gram staining technique
Rectified spirit
95% ethyl alcohol + 3% HCl
100% ethyl alcohol + 3%HCl
35% ethyl alcohol + 3% HCl
Rectified spirit
95% ethyl alcohol + 3% HCl
100% ethyl alcohol + 3%HCl
35% ethyl alcohol + 3% HCl
Answer: 95% ethyl alcohol + 3% HCl
_________ is an agent which kill vegetative cell and also bacterial spores
Germicide
Preservative
Sterilant
Disinfectant
Germicide
Preservative
Sterilant
Disinfectant
Answer: Sterilant
Pasteurization is used for _______
Heat labile fluids
Heat stable fluids
Heat stable powders
All powders
Heat labile fluids
Heat stable fluids
Heat stable powders
All powders
Answer: Heat labile fluids
Hot air oven is used for sterilisation of _________
Heat stable substances
Heat labile fluids
Volatile oil preparations
Rubber gloves
Heat stable substances
Heat labile fluids
Volatile oil preparations
Rubber gloves
Answer: Heat stable substances
Which of the following is a limitation of the autoclave?
It takes too long to sterilize
It will destroy heat-labile materials
It lacks the ability to inactivate viruses
It cannot be used with glassware
It takes too long to sterilize
It will destroy heat-labile materials
It lacks the ability to inactivate viruses
It cannot be used with glassware
Answer: It will destroy heat-labile materials
The time required to kill 90% of the microorganisms in a sample at a specific temperature is the
thermal death temperature
thermal death point
decimal reduction time
D value
thermal death temperature
thermal death point
decimal reduction time
D value
Answer: D value
In the Mac Conkey Agar test, the response showing pink colour around the colony indicates the presence of …………………..
Gram positive bacteria
Gram negative bacteria
Streptococcus bacteria
Mycobacterium Tuberculosis
Gram positive bacteria
Gram negative bacteria
Streptococcus bacteria
Mycobacterium Tuberculosis
Answer: Gram negative bacteria
Acid fast technique was first introduced by
Ziehl and Neelsen
Ziehl and Sorensen
Robert Koch and Alexander
Carl Linnaeus
Ziehl and Neelsen
Ziehl and Sorensen
Robert Koch and Alexander
Carl Linnaeus
Answer: Ziehl and Neelsen
Enterobacter aerogenes produce……………..as end product from the glucose fermentation process.
Organic acid
Strong base
Insoluble impurity
Alkaline residue
Organic acid
Strong base
Insoluble impurity
Alkaline residue
Answer: Organic acid
The decolorizer used in the Gram staining
increases the cell wall permeability
decreases the cell wall permeability
degrades the lipid contents
solidifies the lipid contents
increases the cell wall permeability
decreases the cell wall permeability
degrades the lipid contents
solidifies the lipid contents
Answer: increases the cell wall permeability
Which of the following methods is useful for sterilization of antisera?
Autoclaving
Tyndallisation
Hot air oven
Filtration
Autoclaving
Tyndallisation
Hot air oven
Filtration
Answer: Filtration
Bioburden is ______ number of microorganism present in a product or area
Initial
Final
During process
After the process
Initial
Final
During process
After the process
Answer: Initial
_______ is generated by adding Potassium permanganate with formalin
Formaldehyde
Propiolactone
Ethylene oxide
Halogens
Formaldehyde
Propiolactone
Ethylene oxide
Halogens
Answer: Formaldehyde
_________ is a substance that prevent growth of microorganism but does not kill the microorganism
Preservative
Antibiotic
Germicide
Bactericide
Preservative
Antibiotic
Germicide
Bactericide
Answer: Preservative
Microbiology MCQ with answers:
- Unit I MCQ Introduction to Microbiology, Microscopy, Bacteria
- Unit 2 MCQ Staining techniques, Biochemical tests, and methods of sterilization
- Unit 3 MCQ Fungi and Viruses, Antiseptics and disinfectants
- Unit 4 MCQ Microbiological assay, test, and aseptic area
- Unit 5 MCQ Cell culture, Preservatives, and microbiological spoilage
Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise
MCQ:
BP302T Physical Pharmaceutics I Theory MCQ
BP304T Pharmaceutical Engineering Theory MCQ
Performance Monitoring and Updates in AI-Enabled Medical Devices: FDA’s Guiding Principles
Lifecycle Management in AI-Enabled Medical Devices: FDA’s Comprehensive Framework
Data Management in AI-Enabled Medical Devices: Key to Safety and Effectiveness
