February 22, 2024

Staining techniques, Biochemical tests, and methods of sterilization MCQ

Staining techniques, Biochemical tests, and methods of sterilization MCQ

The SIM broth used in the Indole Product test contains
Tyrosine
Tryptophan
Cysteine
Arginine

Answer: Tryptophan

Mycobacterial cell wall contains a waxy substance composed of 
Picric acid
Mycolic acid
Myristic acid
Carbonic acid

Answer: Mycolic acid

Crystal violet stains the 
Bacterial cytoplasm
Bacterial cell wall
Bacterial nucleus
Bacterial spores

Answer: Bacterial cell wall

Gram staining was developed by 
German bacteriologist
Danish bacteriologist
Russian bacteriologist
American bacteriologist

Answer: Danish bacteriologist

The smear developed in the Acid-fast staining is observed under oil immersion objective lens with a magnification of …………………
10X
100X
1000X
40X

Answer: 100X

Counter stain used in the Acid fast staining technique is 
Methylene blue
Malachite green
Crystal violet
Safranin

Answer: Malachite green

Simple staining technique  can be used to determine 
Reproductive cycle of cells
Presence of Peptidoglycan
Arrangement of bacterial cells only
Different types of bacterial cells

Answer: Arrangement of bacterial cells only

In the Gram staining technique, the counter stain safranin colours the bacterial cells
Red
Pink
Blue
Green

Answer: Pink

………..is used as a dye in simple staining
Gram safranin
Fuchsin stain
Acridine orange
Crystal violet

Answer: Crystal violet

Bacterial cells are stained in _____
Negative staining
Simple staining
Cell wall staining
Capsule staining

Answer: Simple staining

Liquid Parenteral preparations can be sterilized through?
Chemical sterilization
Membrane filtration
Desiccation
Red heat

Answer: Membrane filtration

Which of the following method is used for sterilization of Nichrome wire loop?
Red heat
Membrane filtration
Chemical method
Radiation sterilization

Answer: Red heat

Sterilization is?
Process of growing microbes in the laboratory
Process for the preparation of antibiotics
Killing or removing all the microorganisms
Estimation of potency

Answer: Killing or removing all the microorganisms

Which of the following process does not kill bacterial endospores?
Hot air sterilization
Pasteurization
Autoclave
Incineration

Answer: Pasteurization

The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the
thermal death time
thermal death point
decimal reduction time
thermal death temperature

Answer: thermal death point

Which of the following is bactericidal?
Membrane filtration
Freeze-drying
Ionizing radiation
Incubation

Answer: Ionizing radiation

 ____ is used to prevent infection by killing or inhibiting pathogen growth on animal tissues.
sterilant
disinfectant
adsorbent
antiseptic

Answer: antiseptic

Incineration is method of _______ sterilisation
Moist heat
Dry heat
Filtration
Radiation

Answer: Dry heat

_______ is used to sterilise metallic objects
Incineration
Red heat
Flaming
Sunlight

Answer: Red heat

Indole Production test identifies the following gram negative intestinal bacteria
Escherichia Coli
Streptocococcus
Bacillus antracis
Clostridium

Answer: Escherichia Coli

Reagents used in the Voges-Proskauer test are 
Fehling solution 1 and 2
Kovac’s reagent
Millon’s reagent
A and B Barritt’s reagent

Answer: A and B Barritt’s reagent

Primary stain used in Acid fast staining is
Crystal violet
Carbol Fuschin
Gram safranin
Malachite green

Answer: Carbol Fuschin

Simple staining uses dyes which are ………in nature for staining most of the bacteria
Acidic
Basic
Neutral
Acidic as well as Basic

Answer: Basic

………..is used as decolorizer in the Gram staining technique
Rectified spirit
95% ethyl alcohol + 3% HCl
100% ethyl alcohol + 3%HCl
35% ethyl alcohol + 3% HCl

Answer: 95% ethyl alcohol + 3% HCl

_________ is an agent which kill vegetative cell and also bacterial spores
Germicide
Preservative
Sterilant
Disinfectant

Answer: Sterilant

Pasteurization is used for _______
Heat labile fluids
Heat stable fluids
Heat stable powders
All powders

Answer: Heat labile fluids

Hot air oven is used for sterilisation of _________
Heat stable substances
Heat labile fluids
Volatile oil preparations
Rubber gloves

Answer: Heat stable substances

Which of the following is a limitation of the autoclave?
It takes too long to sterilize
It will destroy heat-labile materials
It lacks the ability to inactivate viruses
It cannot be used with glassware

Answer: It will destroy heat-labile materials

The time required to kill 90% of the microorganisms in a sample at a specific temperature is the
thermal death temperature
thermal death point
decimal reduction time
D value

Answer: D value

In the Mac Conkey Agar test, the response showing pink colour around the colony indicates the presence of …………………..
Gram positive bacteria
Gram negative bacteria
Streptococcus bacteria
Mycobacterium Tuberculosis

Answer: Gram negative bacteria

Acid fast technique was first introduced by 
Ziehl and Neelsen
Ziehl and Sorensen
Robert Koch and Alexander
Carl Linnaeus

Answer: Ziehl and Neelsen

Enterobacter aerogenes produce……………..as end product from the glucose fermentation process.
Organic acid
Strong base
Insoluble impurity
Alkaline residue

Answer: Organic acid

The decolorizer used in the Gram staining 
increases the cell wall permeability
decreases the cell wall permeability
degrades the lipid contents
solidifies the lipid contents

Answer: increases the cell wall permeability

Which of the following methods is useful for sterilization of antisera?
Autoclaving
Tyndallisation
Hot air oven
Filtration

Answer: Filtration

Bioburden is ______ number of microorganism present in a product or area
Initial
Final
During process
After the process

Answer: Initial

 _______ is generated by adding Potassium permanganate with formalin
Formaldehyde
Propiolactone
Ethylene oxide
Halogens

Answer: Formaldehyde

 _________ is a substance that prevent growth of microorganism but does not kill the microorganism
Preservative
Antibiotic
Germicide
Bactericide

Answer: Preservative


Microbiology MCQ with answers:


Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem IIIS Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I TheoryBP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology TheoBP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II PracticalBP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

MCQ:

BP302T Physical Pharmaceutics I Theory MCQ

BP304T Pharmaceutical Engineering Theory MCQ

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