Pharmaceutical Microbiology Theory syllabus, Notes, Books, PDF, Download
Unit I
Introduction and Scope of Microbiology
History of microbiology, (MCQ Video Link: Click here)
Introduction to Prokaryotes and Eukaryotes
Study of ultra-structure of bacteria
Nutritional requirements,
Raw materials used for culture media (MCQ Video Link: Click here) (Video link: Click here)
Physical parameters for growth, Growth curve (MCQ Video Link: Click here)
Isolation of pure cultures (MCQ Video Link: Click here)
Preservation methods for bacteria
Cultivation of anaerobes,
Quantitative measurement of bacterial growth (total & viable count).
Microscopy: Study of different types of phase contrast microscopy, darkfield microscopy, and electron microscopy.
Unit I MCQ Introduction to Microbiology, Microscopy, Bacteria
Unit II
Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and
Biochemical tests (IMViC).
Study of principle, procedure, merits, demerits, and applications of physical, chemical gaseous, radiation, and mechanical method of sterilization.
(MCQ Video Link: Sterilisation Part I, Sterilisation Part II)
Evaluation of the efficiency of sterilization methods.
Equipment employed in large-scale sterilization.
Sterility indicators.
Unit 2 MCQ Staining techniques, Biochemical tests, and methods of sterilization
Unit III
Fungi: Study of morphology, classification, reproduction/replication, and cultivation
Study of morphology, classification, reproduction/replication, and cultivation of Viruses
Classification and mode of action of disinfectants
Factors influencing disinfection, antiseptics, and their evaluation.
For bacteriostatic and bactericidal actions: Evaluation of bactericidal & Bacteriostatic.
Sterility testing of products (solids, liquids, ophthalmic, and other sterile products) according to IP, BP, and USP.
Unit 3 MCQ Fungi and Viruses, Antiseptics and disinfectants
Unit IV
Designing of aseptic area, and laminar flow equipment;
Different sources of contamination in an aseptic area
Methods of prevention of contamination
Clean area classification.
Antibiotics: Principles and methods of microbiological assays
Vitamins: Principles and methods of microbiological assays
Amino acids: Principles and methods of microbiological assays
Methods for standardization of antibiotics, vitamins, and amino acids.
Assessment of a new antibiotic
Unit 4 MCQ Microbiological assay, test, and aseptic area
Unit V
Types of spoilage,
Factors affecting the microbial spoilage of pharmaceutical products, sources, and
Types of microbial contaminants,
Assessment of microbial contamination and spoilage.
Preservation of pharmaceutical products using antimicrobial agents,
Evaluation of microbial stability of formulations.
Growth of animal cells in culture, the general procedure for cell culture,
Primary, established and transformed cell cultures.
Application of cell cultures in the pharmaceutical industry and research
Unit 5 MCQ Cell culture, Preservatives, and microbiological spoilage
Pharmaceutical Microbiology Recommended Books (Latest Edition)
- W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific
publications, Oxford London. - Prescott and Dunn., Industrial Microbiology, 4th edition, CBS Publishers & Distributors,
Delhi. - Pelczar, Chan Kreig, Microbiology, Tata McGraw Hill edn.
- Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
- Rose: Industrial Microbiology.
- Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
- Cooper and Gunn’s: Tutorial Pharmacy, CBS Publisher and Distribution.
- Peppler: Microbial Technology.
- I.P., B.P., U.S.P.- latest editions.
- Ananthnarayan: Text Book of Microbiology, Orient-Longman, Chennai
- Edward: Fundamentals of Microbiology.
- N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
- Bergey’s manual of systematic bacteriology, Williams and Wilkins- A Waverly company
Suggested readings:
b pharmacy syllabus * Pharmaceutics I practical * Social Pharmacy Theory *
