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Study of stereotype and anti-catatonic activity of drugs on rats/mice

BP408P Pharmacology I Practical / S Y B Pharmacy Notes

Aim:

To study drug (phenothiazines)- induced catatonia (extrapyramidal side effect in rats

To study the anticatatonic (antiparkinsonian) effect of scopolamine.

Principle:

Phenothiazine and butyrophenone type of antipsychotic drugs are known to produce extrapyramidal side-effects in man .these effects , such as akinesia, rigidity and tremors, are called parkinson’s- like because in parkinson’s disease the major clinical symptoms include difficulty to move and change posture (akinesia and rigidity) and tremors. These effects of antipsychotic drugs are due to excessive blockade of dopamine receptors in the extrapyramidal motor system. Therefore, phenothiazines (choloropromazine or perphenazine) are commonly used to produce parkinson’s-like extrapyramidal symptoms in laboratory animals and to study anti-parkinsonism drugs.The students are advised to know the pharmacology of anti-parkinsonism drugs before performing this experiment.

Requirements

Animal:   Rats (150-200 g)

Drugs: Perphenazine (dose: 5 mg/kg ip; prepare a stock solution containing 1 mg/ml of the drug and inject 0.5 ml/100g body weight of the animal). Scopolqmine (dose: 2 mg/kg, ip prepare a stock solution containing 0.4 mg/ml of the drug and inject 0.5 ml/100 g body weight of the animal)

Equipment    two wooden blocks, one being 3 cm high and the other 9 cm high

Procedure

  • Weigh and number the animal. Divide the animals into two groups, one for control (for study of perphenazine effect) and the other for studying the effects of scopolamine. Each group should consist of at least 5 animals.
  • Inject perphenazine to control animals. observe the severity of catatonic response (fig 5.14) as follows:
  • Observe the severity of catatonia at 5,15,30,45,60,90 and 120 min after perphenazine.
  • To the second group inject scopolamine and after 30 minutes inject perphenazine into these animals already treated with scopolamine. Observe and score the severity of catatonic as in step 2.
  • Compare the onset and severity of catatonic response in both groups. Plot a graph, time along the x-axis. Note the difference in the onset and severity of catatonic response in both groups.

Pharmacology I Practical

  1. Introduction to experimental pharmacology.
  2. Commonly used instruments in experimental pharmacology.
  3. Study of common laboratory animals.
  4. Maintenance of laboratory animals as per CPCSEA guidelines.
  5. Common laboratory techniques.
  6. Study of different routes of drugs administration in mice/rats.
  7. Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
  8. Effect of drugs on ciliary motility of frog oesophagus
  9. Effect of drugs on rabbit eye.
  10. Effects of skeletal muscle relaxants using rota-rod apparatus.
  11. Effect of drugs on locomotor activity using actophotometer.
  12. Anticonvulsant effect of drugs by MES and PTZ method.
  13. Study of stereotype and anti-catatonic activity of drugs on rats/mice.
  14. Study of anxiolytic activity of drugs using rats/mice.
  15. Study of local anesthetics by different methods

Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem IIIS Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I TheoryBP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology TheoBP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II Practical BP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

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