Study of stereotype and anti-catatonic activity of drugs on rats/mice
BP408P Pharmacology I Practical / S Y B Pharmacy Notes
Aim:
To study drug (phenothiazines)- induced catatonia (extrapyramidal side effect in rats
To study the anticatatonic (antiparkinsonian) effect of scopolamine.
Principle:
Phenothiazine and butyrophenone type of antipsychotic drugs are known to produce extrapyramidal side-effects in man .these effects , such as akinesia, rigidity and tremors, are called parkinson’s- like because in parkinson’s disease the major clinical symptoms include difficulty to move and change posture (akinesia and rigidity) and tremors. These effects of antipsychotic drugs are due to excessive blockade of dopamine receptors in the extrapyramidal motor system. Therefore, phenothiazines (choloropromazine or perphenazine) are commonly used to produce parkinson’s-like extrapyramidal symptoms in laboratory animals and to study anti-parkinsonism drugs.The students are advised to know the pharmacology of anti-parkinsonism drugs before performing this experiment.
Requirements
Animal: Rats (150-200 g)
Drugs: Perphenazine (dose: 5 mg/kg ip; prepare a stock solution containing 1 mg/ml of the drug and inject 0.5 ml/100g body weight of the animal). Scopolqmine (dose: 2 mg/kg, ip prepare a stock solution containing 0.4 mg/ml of the drug and inject 0.5 ml/100 g body weight of the animal)
Equipment two wooden blocks, one being 3 cm high and the other 9 cm high
Procedure
- Weigh and number the animal. Divide the animals into two groups, one for control (for study of perphenazine effect) and the other for studying the effects of scopolamine. Each group should consist of at least 5 animals.
- Inject perphenazine to control animals. observe the severity of catatonic response (fig 5.14) as follows:
- Observe the severity of catatonia at 5,15,30,45,60,90 and 120 min after perphenazine.
- To the second group inject scopolamine and after 30 minutes inject perphenazine into these animals already treated with scopolamine. Observe and score the severity of catatonic as in step 2.
- Compare the onset and severity of catatonic response in both groups. Plot a graph, time along the x-axis. Note the difference in the onset and severity of catatonic response in both groups.
- Introduction to experimental pharmacology.
- Commonly used instruments in experimental pharmacology.
- Study of common laboratory animals.
- Maintenance of laboratory animals as per CPCSEA guidelines.
- Common laboratory techniques.
- Study of different routes of drugs administration in mice/rats.
- Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
- Effect of drugs on ciliary motility of frog oesophagus
- Effect of drugs on rabbit eye.
- Effects of skeletal muscle relaxants using rota-rod apparatus.
- Effect of drugs on locomotor activity using actophotometer.
- Anticonvulsant effect of drugs by MES and PTZ method.
- Study of stereotype and anti-catatonic activity of drugs on rats/mice.
- Study of anxiolytic activity of drugs using rats/mice.
- Study of local anesthetics by different methods
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