November 28, 2023

Effect of drugs on rabbit eye: Pharmacology Lab Manual

Effect of drugs on rabbit eye: Pharmacology Lab Manual

BP408P Pharmacology I Practical / S Y B Pharmacy Notes


To study the Mydriatic effect of topically applied atropine on rabbit eye


A large number of drugs are used for their local action in the eye as eye drops or eye ointments. Most of these drugs belongs to anti microbial, autonomic or local anaesthetic group. The eye is supplied by both sympathetic and parasympathetic nerves. The superior palpebral muscle and the dilator papillae of the iris have sympathetic supply. The sphincter pupillae of the iris has parasympathetic supply which exercises dominant control. The ciliary muscle is also supplied by parasympathetic nerve and when it contracts, the ciliary body is moved inwards and forwards. Because of the lens bulges forward and the eye is accommodated for near vision. The opposite effect is produced by the relaxation of ciliary muscle resulting in paralysis of accommodation. When the pupil dilates, the iris folds back near the opening of the canal of schlemn and the drainage of aqueous humour is decreased thereby increasing intraocular pressure. constriction of the pupil, by opposite action, will increase drainage and reduce intraocular pressure.

Topically applied drugs can affect the eye by changing conjuctival congestion, papillary size, light relex, corneal sensitivity and intraocular pressure. however the effects of drugs on papillary size, light reflex and corneal reflex can be easily studied by the students. the pupillary size can be measured by placing a transparent plastic scale in front of the eye but as close as possible. Light reflex is elicited by directing the light of a torch towards the pupil. The sensitivity of the cornea is tested by gently touching the cornea with a fine cotton swab stick from the side and not from the front of the eye. This elicits corneal reflex which manifests as blinking of the crystals.


Animal : Rabbit(2-5kg)

Drugs: Atropine (1%w/v) & Physostigmine (1%w/v)

Equipment: rabbit holder, pen torch


  1. Place the rabbit in the rabbit holder by keeping the head outside
  2. Observe size of the pupil in both the eyes
  3. Examine the effect of light reflex by holding the torch in front of the eye moving light beam to and fro
  4. Examine the corneal reflex by touching a side of the cornea with a cotton swab.
  5. Instil a few drops of atropine solution in the conjunctiva over a period of 8-10 min in the right eye of the rabbit would serve as control. Instil normal saline in the left eye.
  6. Record  the  pupillary  size,  light  reflex  and  corneal  refkex  after  ten  minutes  of  drug initialisation and tabulate the observation.
  7. Repeat the experiments with physostigmine and ephedrine



Pharmacology I Practical

  1. Introduction to experimental pharmacology.
  2. Commonly used instruments in experimental pharmacology.
  3. Study of common laboratory animals.
  4. Maintenance of laboratory animals as per CPCSEA guidelines.
  5. Common laboratory techniques.
  6. Study of different routes of drugs administration in mice/rats.
  7. Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
  8. Effect of drugs on ciliary motility of frog oesophagus
  9. Effect of drugs on rabbit eye.
  10. Effects of skeletal muscle relaxants using rota-rod apparatus.
  11. Effect of drugs on locomotor activity using actophotometer.
  12. Anticonvulsant effect of drugs by MES and PTZ method.
  13. Study of stereotype and anti-catatonic activity of drugs on rats/mice.
  14. Study of anxiolytic activity of drugs using rats/mice.
  15. Study of local anesthetics by different methods

Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem IIIS Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I TheoryBP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology TheoBP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II Practical BP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

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