September 30, 2023

Study of anxiolytic activity of drugs using rats/mice. (elevated zero maze)

Study of anxiolytic activity of drugs using rats/mice. (elevated zero maze)

BP408P Pharmacology I Practical / S Y B Pharmacy Notes


To study the anxiolytic activity of diazepam in mice using elevated zero maze


The elevated zero maze test is a behavioural test of anxiety based on the naturalistic tendency of rodents to avoid open and elevated areas. It is similar to the more widely used elevated plus maze, except that the open and closed arms are arranged circularly, thus eliminating the centre area which removes ambiguity in the interpretation of time spent in the central square of the traditional design. The maze is an elevated, white or black, annual having an outer diameter of 45 cm and an inner diameter of 30 cm. The runway ring where the mouse can explore is 6cm in width and is divided into 4 quadrants, 2 opposing “open” quadrants without walls and 2 opposing closed quadrants. The model is widely used for the elevation of anxiolytic agents belonging to a chemically different class of drugs.


Animal:          Mice (20-25gm)

Drugs:  Diazepam (dose 2 mg/kg. i.p)

Equipment:   Zero mazes with the dimension


Weigh and number the animals . divide them into two groups each consisting of minimum 6 mice. One group is used as control and other drugs treatment.

Place the animal individually in the open arm facing towards the closed arm. Start the stop watch and note the following parameters for a period of six minutes.

  • Latency to enter the open arm
  • Average time each animals spends in the open arm
  • Total number of entries in the open arm
  • Stretching into open quadrants

Clean the maze thoroughly using tissue paper after each trail.

Inject  diazepam to the test  group. After 30 min place the animals individually as described.

Pharmacology I Practical

  1. Introduction to experimental pharmacology.
  2. Commonly used instruments in experimental pharmacology.
  3. Study of common laboratory animals.
  4. Maintenance of laboratory animals as per CPCSEA guidelines.
  5. Common laboratory techniques.
  6. Study of different routes of drugs administration in mice/rats.
  7. Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
  8. Effect of drugs on ciliary motility of frog oesophagus
  9. Effect of drugs on rabbit eye.
  10. Effects of skeletal muscle relaxants using rota-rod apparatus.
  11. Effect of drugs on locomotor activity using actophotometer.
  12. Anticonvulsant effect of drugs by MES and PTZ method.
  13. Study of stereotype and anti-catatonic activity of drugs on rats/mice.
  14. Study of anxiolytic activity of drugs using rats/mice.
  15. Study of local anesthetics by different methods

Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem IIIS Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I TheoryBP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology TheoBP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II Practical BP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

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