Effects of skeletal muscle relaxants using rota-rod apparatus

Effects of skeletal muscle relaxants using rota-rod apparatus

BP408P Pharmacology I Practical / S Y B Pharmacy Notes

Aim:

To study the muscle relaxant property of diazepam in mice using rota rod apparatus

Principle:

One of the important pharmacological action of anti anxiety agents of benzo diazepam class of drugs is muscle relaxaing property. The skeletal muscle relaxation together with taming or calming effect, these agents reduce anxiety and tension. The loss of muscle grip is an indication of muscle relaxation. This effect can be easily studied in animals using inclined plane or rotating rods.

The difference in the fall off time from the rotating rod between the control and diazepam treated animal is taken as an index of muscle relaxation . The angle of the slope of the inclined plane, or the rate of rotation of the rod should be adjusted such that a normal mouse can stay on the plane or on the rod for an appreciable period of time.

Requirement:

Animal: Mice (20-25gm)

Drugs: Diazepam of the drug and inject 1ml/100g of body weight of the mouse.

Equipment: Rota rod apparatus

Procedure:

1. Weigh the animals and number them.
2. Turn on the instrument select an appropriate speed (20-25rpm)
3. Place the animal one by one on the rotating rod. One can place more than one mouse. Note down the fall off time when the mouse falls from the rotating rod. A normal mouse generally falls off within 3-5 min
4. Inject diazepam to all animals. After 30 min repeat the experiments as done in step 3 . note the fall off time.
5. Compare the fall off time of animals before and after diazepam treatment.

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Pharmacology I Practical

1. Introduction to experimental pharmacology.
2. Commonly used instruments in experimental pharmacology.
3. Study of common laboratory animals.
4. Maintenance of laboratory animals as per CPCSEA guidelines.
5. Common laboratory techniques.
6. Study of different routes of drugs administration in mice/rats.
7. Study effect of hepatic microsomal enzyme inducers on phenobarbitone sleeping time in mice.
8. Effect of drugs on ciliary motility of frog oesophagus
9. Effect of drugs on rabbit eye.
10. Effects of skeletal muscle relaxants using rota-rod apparatus.
11. Effect of drugs on locomotor activity using actophotometer.
12. Anticonvulsant effect of drugs by MES and PTZ method.
13. Study of stereotype and anti-catatonic activity of drugs on rats/mice.
14. Study of anxiolytic activity of drugs using rats/mice.
15. Study of local anesthetics by different methods

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Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

S Y B Pharm Sem III	S Y B Pharm Sem IV
BP301T Pharmaceutical Organic Chemistry II Theory	BP401T Pharmaceutical Organic Chemistry III Theory
BP302T Physical Pharmaceutics I Theory	BP402T Medicinal Chemistry I Theory
BP303T Pharmaceutical Microbiology Theo	BP403T Physical Pharmaceutics II Theory
BP304T Pharmaceutical Engineering Theory	BP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II Practical	BP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I Practical	BP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology Practical	BP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering Practical	BP408P Pharmacology I Practical
	BP409P Pharmacognosy I Practical

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Suggested readings:

• BP101T Human Anatomy and Physiology I Theory
• Commonly used instruments in experimental pharmacology
• ER20-12T Pharmaceutical Chemistry Theory D Pharm
• Microscopic study of muscular and nervous tissue
• Structure and functions of Cell Human Anatomy and Physiology Notes