Assay of Chlorpromazine: Medicinal Chemistry Practical
AIM:To carry out the Assay of Chlorpromazine.
REFERENCE:Indian Pharmacopoeia 2018 page no:1600-01.
Perchloric acid (0.1M),Chlorpromazine, mercuric acetate solution(5% w/v in acetic acid),crystal violet solution (0.2%w/v in acetic acid),acetone,methyl orange indicator, conical flask, burette, beaker, potassium hydrogen phthalate, glacial acetic acid, crystal violet indicator.
Chlorpromazine is estimated by non-aqueous titration which is suitable for titration of weak acid and weak base. In this non aqueous solvent like perchloric acid is utilized as a titrant and methyl orange is used as an indicator. Mercuric acetate is added in the non-aqueous titration in order to remove the chloride ions. So as to prevent the interference of the chloride ion released by the titrant. The mercuric acetate replaces the halide ion in chlorpromazine with acetate ion which is a strong base. The end point is indicated by appearance of blue colour.
- STANDARDISATION OF PERCHLORIC ACID (0.1N)
Dissolved 0.5g of potassium hydrogen phthalate in 25ml of glacial acetic acid and added few drops of 5%w/v crystal violet indicator. Titrated the solution with 0.1N perchloric acid till blue green colour appears.
ASSAY OF CHLORPROMAZINE
Weighed accurately about 0.6g and dissolved in 200 ml of acetone. Added 15ml of mercuric acetate solution. Titrated with 0.1M perchloric acid, using a saturated solution of methyl orange in acetone as indicator. Perform a blank determination and make a necessary correction.
Each ml of 0.1M perchloric acid equivalent to 0.03553g of C17H19ClN2S,HCl
The given sample contains …………………. mg of chlorpromazine
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