May 19, 2024

Guidelines on Dosage Calculation and Stock Solution Preparation in Experimental Animals Studies

Guidelines on Dosage Calculation and Stock Solution Preparation in Experimental Animals Studies

Aim

To prepare stock solution and calculate dosage calculations in experimental animals studies (or) Preclinical studies

Introduction

Experimental animals are very important tool in non-human research models

Dosage calculations and stock solution preparation based on dosage rationable formula are prerequisites to drug administration in experimental animals

However, drug dosage calculations and stock solutions preparations are not clearly explained in most scientific literature.

Vehicle of choice, drugs dissolutions & volume selections rationale:

A vehicle is any substance that acts as when drug is administered

Vehicle which is an essential consideration in all animal research should be biologically inert, it has no toxic effects on animals influence the results obtained for the compound under investigations.

Eg-water, normal saline (0.9% nacl), 50% polyethylene glycol, 5-10% tween 80,0.25% methyl cellulose (or) carboxy methyl cellulose.

In most researches involving experimental animals dosage are usually calculated from stock solution of the test drugs dissolved in vehicle.

According to OECD’s (organization of economic corporation and development) guidelines, dosage of drug should be constituted in an appropriate volume not usually exceeding 10 ml/kg body weight of experimental animals for non aqueous solvent in oral route of administration.

However the aqueous solvents 20ml/kg body weight can be considered large dose volumes. Can cause unnecessary stress to animals large dose volumes. Can cause unnecessary stress to animal can also overload the stomach capacity and pass immediately into the small bowel or can result in passive reflux in stomach, aspiration, pneumonia, pharynageal, esophageal and gastrin irritation or injury with structure formation.

However, highly viscous drug solutions should be diluted whenever possible, for ease of adininistation.

However, final dilution valume should be not exceed 20ml/kg. based on 10ml/kg valume selection, required dose valume for a 100gm rat can be calculated as follows 100g/1000gx10ml=ml

NB-1g=100g

Based on 20ml/kg volume selection, required dose volume for a 100g not can be calculated as follows.

100g/1000gx20ml=2ml


Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem VT Y B Pharm Sem VI
BP501T Medicinal Chemistry II TheoryBP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy TheoryBP602T Pharmacology III Theory
BP503T Pharmacology II TheoryBP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II TheoryBP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence TheoryBP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I PracticalBP606T Quality Assurance Theory
BP507P Pharmacology II PracticalBP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II PracticalBP608P Pharmacology III Practical
BP609P Herbal Drug Technology Practical

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