Test for pyrogens rabbit method: Pharmacology Practical Lab Manual PDF
To prepare and submitted preliminary test (sham test) and rabbit pyroxene test.
Groups of three healthy nature rabbits are chosen. Accurate thermometers are inserted into the rectum of the rabbits to record their body temperature. Test solutions are warmed to 370C prior to injection. Rabbit temperatures are recorded at 30min intervals between 1 and 3hr.
Inject the solution (38.50C) under examination slowly into the marina (vein of the ear of each rabbit) over a period of exceeding 4 minutes unless otherwise prescribed in the monograph. Record the temperature of each animal at half-hourly intervals for 3 hours after the injection.
The difference between the initial temp and the maximum temperature which is the highest temp recorded for rabbits taken to be its response.
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