May 19, 2024

Insulin hypoglycemic effect in the rabbit: Pharmacology III Practical

Insulin hypoglycemic effect in the rabbit: Pharmacology III Practical

Aim:

To determine the insulin hypoglycemic effect in rabbits.

Animal required:

Healthy rabbits weighing 1800-3000gms.

Drugs :

20 units of insulin preparation. One unit contains 0.04082mg of insulin.

Reagents:

Normal saline, HCL, 0.5% phenol, 1.4-1.8% glycerin.

Procedure:

Select healthy rabbits weighing 1800-3000gems for the study. They should be maintained on a uniform diet for 7 days. Fast the animals for 18hrs with no access to water before starting the procedure. Select three animals for the study and inject 1 unit/ml of insulin.

Prepare drug solution freshly. Weigh 20 units of insulin accurately and dissolve it in normal saline. Acidify the solution by using HCL to pH 2.5. Add 0.5% of phenol as a preservative and 1.4-1.8% of glycerin and make the final volume to 20% unit/ml of solution.

Withdraw 2ml of blood from the marginal ear vein of each rabbit and estimate blood glucose level by using a suitable biochemical method and the concentration of glucose can be noted down an initial blood glucose level. Then injection (1 unit/ml) to the animals and check the blood sugar level for up to 5 hours at the interval of 1 hour each and determine blood glucose levels as finding blood glucose levels.

Report:

The mean percentage decrease of blood glucose levels at different time intervals determines the effect of insulin.


Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem VT Y B Pharm Sem VI
BP501T Medicinal Chemistry II TheoryBP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy TheoryBP602T Pharmacology III Theory
BP503T Pharmacology II TheoryBP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II TheoryBP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence TheoryBP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I PracticalBP606T Quality Assurance Theory
BP507P Pharmacology II PracticalBP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II PracticalBP608P Pharmacology III Practical
BP609P Herbal Drug Technology Practical

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