Insulin hypoglycemic effect in the rabbit: Pharmacology III Practical

Aim:

To determine the insulin hypoglycemic effect in rabbits.

Animal required:

Healthy rabbits weighing 1800-3000gms.

Drugs :

20 units of insulin preparation. One unit contains 0.04082mg of insulin.

Reagents:

Normal saline, HCL, 0.5% phenol, 1.4-1.8% glycerin.

Procedure:

Select healthy rabbits weighing 1800-3000gems for the study. They should be maintained on a uniform diet for 7 days. Fast the animals for 18hrs with no access to water before starting the procedure. Select three animals for the study and inject 1 unit/ml of insulin.

Prepare drug solution freshly. Weigh 20 units of insulin accurately and dissolve it in normal saline. Acidify the solution by using HCL to pH 2.5. Add 0.5% of phenol as a preservative and 1.4-1.8% of glycerin and make the final volume to 20% unit/ml of solution.

Withdraw 2ml of blood from the marginal ear vein of each rabbit and estimate blood glucose level by using a suitable biochemical method and the concentration of glucose can be noted down an initial blood glucose level. Then injection (1 unit/ml) to the animals and check the blood sugar level for up to 5 hours at the interval of 1 hour each and determine blood glucose levels as finding blood glucose levels.

Report:

The mean percentage decrease of blood glucose levels at different time intervals determines the effect of insulin.

---

Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem V	T Y B Pharm Sem VI
BP501T Medicinal Chemistry II Theory	BP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy Theory	BP602T Pharmacology III Theory
BP503T Pharmacology II Theory	BP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II Theory	BP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence Theory	BP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I Practical	BP606T Quality Assurance Theory
BP507P Pharmacology II Practical	BP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II Practical	BP608P Pharmacology III Practical
	BP609P Herbal Drug Technology Practical

---

Suggested readings:

• Insulin Production rDNA technology notes
• BP608P Pharmacology III Practical
• Effect of drugs on rabbit eye: Pharmacology Lab Manual
• Diabetes by Neutraceuticals Notes
• ER20-21P Pharmacology Practical D Pharm

Recommended readings:

• human anatomy and physiology
• biosensors in Pharmaceutical Industries

---

---