February 19, 2025

Demonstration of total blood count by cell analyzer

Demonstration of total blood count by cell analyzer

There are three types of cells in the bloodstream namely leucocytes (white blood cells), erythrocytes (red blood cells), and thrombocytes (platelets). The complete blood count is the most common blood investigation advised by the doctor to know the overall health of the subject.

The hematology analyzers are used to detect the total count of blood cells and also to diagnose diseases of the blood cells and monitor the health condition of the patient. Some of the complex analyzers also measure the cell morphology and help in the diagnosis of rare blood disorders. The technologies used in hematology analyzers are electrical impedance, flow cytometry, and fluorescent flow cytometry.

The aim of the experiment is to demonstrate total blood count by a cell analyzer.

REQUIREMENTS

Subjects: Human

Apparatus: Hematology analyzer

Blood sample

PROCEDURE

The subject is either in a sitting or lying down position. The suitable vein is punctured by placing a tourniquet 3- 4 inches above the puncture site. The blood is collected and sent for analysis.

The following technologies are used in hematology analyzers.

Electrical impedance

This is known as the Coulter principle, used in most analyzers. The blood is passed between two electrodes through a narrow aperture which can pass a single cell at a time. The change in impedance is proportional to the cell volume, which in turn measures the cell count and volume.2

Flow cytometry

It is used to determine 5-part WBC differentials. The single stream of light passing through the laser beam measures cell granularity, diameter, and inner complexity. The addition of fluorescent reagents to flow cytometry renders it fluorescent flow cytometry which measures specific cell populations namely platelets, nucleated RBCs, and reticulocytes.2

CONCLUSION

Complete blood count analysis is used in diagnosing diseases, monitoring health conditions, and knowing the prognosis of medical treatments.


BP207P Human Anatomy and Physiology II Practical

To study the integumentary and special senses using specimens, models, etc.,
To study the nervous system using specimens, models, etc.,
To study the endocrine system using specimens, models, etc
To demonstrate the general neurological examination
To demonstrate the function of olfactory nerve
To examine the different types of taste.
To demonstrate the visual acuity
To demonstrate the reflex activity
Recording of body temperature
Determination of tidal volume and vital capacity.
Study of digestive, respiratory, cardiovascular systems, urinary and reproductive systems with the help of models, charts and specimens.
Recording of the basal mass index.
Study of family planning devices and pregnancy diagnosis test.
Demonstration of total blood count by cell analyzer
Permanent slides of vital organs and gonads


First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

F Y B Pharm Sem-IF Y B Pharm Sem-II
BP101T Human Anatomy and Physiology I TheoryBP201T Human Anatomy and Physiology II – Theory
BP102T Pharmaceutical Analysis I TheoryBP202T Pharmaceutical Organic Chemistry I Theory
BP103T Pharmaceutics I TheoryBP203T Biochemistry – Theory
BP104T Pharmaceutical Inorganic Chemistry TheoryBP204T Pathophysiology – Theory
BP105T Communication skills TheoryBP205T Computer Applications in Pharmacy Theory
BP106RBT Remedial BiologyBP206T Environmental sciences – Theory
BP106RMT Remedial Mathematics TheoryBP207P Human Anatomy and Physiology II Practical
BP107P Human Anatomy and Physiology PracticalBP208P Pharmaceutical Organic Chemistry I Practical
BP108P Pharmaceutical Analysis I PracticalBP209P Biochemistry Practical
BP109P Pharmaceutics I PracticalBP210P Computer Applications in Pharmacy Practical
BP110P Pharmaceutical Inorganic Chemistry Practical
BP111P Communication skills Practical
BP112RBP Remedial Biology Practical

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