Recombinant DNA technology: Pharmaceutical Biotechnology Theory Notes

Recombinant DNA technology

Pharmaceutical Biotechnology Theory Notes

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STEPS INVOLVED IN THE PROCESS

Isolation

Isolate the gene of interest (foreign gene) from various sources for different applications. The cell or organism from which the required gene is taken is called a ‘donor’.

Fragmentation

The gene is fragmented and isolated from the donor using restriction enzymes.

Addition

Adding the fragments with the desired gene into the ‘cloning vector’ (a plasmid, cosmid, or phage DNA), so as to develop recombinant DNA or chimeric DNA. Vectors are used to carry the desired gene from the donor to the hosts in which it will multiply.

Ligation

The desired gene is inserted into the vector using suitable ligase enzymes which can be selected depending upon the type of bond to be formed between the foreign and vector DNA.

Transformation

Introducing the recombinant vector into a suitable host cell in which it is able to multiply without interfering with the host functioning.

Replication or multiplication

Host has the ability to multiply and produce the required protein or any other substance for which it is formulated. Hosts are used for getting large quantity of product from the foreign gene which is our objective.

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Overview Recombinant DNA technology

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Application of genetic engineering in medicine

1. Biotechnology plays an important role in the development of successful fermentation technology, producing recombinant proteins and peptides.
2. These products have justified their uses as absolute supply, relative supply, safely specificity and economics.
3. Naturally derived biological products, such as hGH, insulin, etc., of human origin are prone to contamination from their source.
4. The rDNA products are safe as their use is concerned.
5. In case of diagnostic products i.e. antibodies, the most significant aspect is specificity.
6. The recombinant-derived proteins provide a higher level of sensitivity and specificity than had been available previously.
7. Proteins are produced in minute quantity by living organisms, the genes for these have been identified, cloned and expressed at a high level in host cells.
8. Production of insulin.
9. Production of human growth hormone.
10. Production of interferon.

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Reference:

SIMPLIFIED CONCEPTS OF BIOTECHNOLOGY By, Dr. Pramod Kadu & Ms. Suchita Vishwakarma

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Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem V	T Y B Pharm Sem VI
BP501T Medicinal Chemistry II Theory	BP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy Theory	BP602T Pharmacology III Theory
BP503T Pharmacology II Theory	BP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II Theory	BP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence Theory	BP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I Practical	BP606T Quality Assurance Theory
BP507P Pharmacology II Practical	BP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II Practical	BP608P Pharmacology III Practical
	BP609P Herbal Drug Technology Practical

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Suggested readings:

• Polymerase chain reaction PCR / Gene amplification
• BP605T Pharmaceutical Biotechnology Theory Books, PDF Notes, Download
• Introduction to Biotechnology with reference to Pharmaceutical Sciences
• BP508P Pharmacognosy II Practical
• BP609P Herbal Drug Technology Practical

Recommended readings:

• Cloning vectors
• DNA ligase
• Restriction endonucleases

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