February 22, 2024

Preparation of Ascorbic Acid injection

Preparation of Ascorbic Acid injection

BP506P Industrial Pharmacy I Practical


Ascorbic acid (vitamin c) is a water-soluble vitamin. It occurs as a white or slightly yellow

Crystal or powder with a slight acidic taste. It is an antiscorbutic product. On exposure to Light, it gradually darkens. In the dry state, it is reasonably stable in air, but in solution it Rapidly oxidizes. Ascorbic acid is freely soluble in water; sparingly soluble in alcohol;

Insoluble in chloroform, in ether, and in benzene. The chemical name of ascorbic acid is lascorbic acid. The empirical formula is c6h806, and the molecular weight is 176.13. The ascorbic acid injection is a sterile solution.

Each ml contains: Edetate disodium 0.025% in water for injection qs. Prepared with the aid of sodium Bicarbonate. Sodium hydroxide and/or hydrochloric acid may have been used to adjust ph.

IngredientsQuantity given/Ampoule
Ascorbic acid 
Disodium edentate 
Sodium Hydroxide 
Sodium bicarbonate 
Water  for  injection q.s 

Apparatus:      beaker,      measuring    cylinder,      syringe,    needle,    ampoules,whatman    filter paper/membrane filter, Ph meter.

Procedure:

  • Type I glass ampoules are selected and clean. The formulation is carried out in clean area.
  • Weigh accurately ascorbic acid.
  • Dissolve ascorbic acid in water for injection in a beaker with application of heat then add calcium d saccharate to it.
  • adjust the ph in between 6-8 with 10% sodium hydroxide solution.
  • Keep the solution for cooling.
  • cooling  filter  the  solution  through  whatman  filter  paper/  0.45  μmmembrane  filter  to remove

any particulate matter.

  • Fill the prepared injection in ampoules (type i) with the help of syringe.
  • Seal the ampoules by pull seal technique.
  • Sterilize the ampoules by autoclaving at 121°c for 30 minutes.

Packing:

in a suitable ampoule box the ampoules are packed and labeled.

Storage:

store below 40 °c, preferably between 2∘ and 8 °c, unless otherwise specified by manufacturer. No preservative added. Store in a well closed container and use only if solution is clear and seal intact.

Uses:

Vitamin C is recommended for the prevention and treatment of scurvy

Quality Control Test:

  1. Particulate test
  2. Leaker test
  3. Sterility test
  4. Content uniformity test
  5. Pyrogen test

Result:

Ascorbic acid injection ampoules prepared and submitted


Industrial Pharmacy I Practical

  1. Preformulation studies on paracetamol/aspirin/or any other drug
  2. Preparation and evaluation of Paracetamol tablets
  3. Preparation and evaluation of Aspirin tablets
  4. Coating of tablets- film coating of tables/granules
  5. Preparation and evaluation of Tetracycline capsules
  6. Preparation of Calcium Gluconate injection
  7. Preparation of Ascorbic Acid injection
  8. Quality control test of (as per IP) marketed tablets and capsules
  9. Preparation of Eye drops/ and Eye ointments
  10. Preparation of Creams (cold / vanishing cream)
  11. Evaluation of Glass containers (as per IP)

Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem VT Y B Pharm Sem VI
BP501T Medicinal Chemistry II TheoryBP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy TheoryBP602T Pharmacology III Theory
BP503T Pharmacology II TheoryBP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II TheoryBP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence TheoryBP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I PracticalBP606T Quality Assurance Theory
BP507P Pharmacology II PracticalBP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II PracticalBP608P Pharmacology III Practical
BP609P Herbal Drug Technology Practical

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