2.2 Pharmaceutical microbiology Pharm D Syllabus, Notes, PDF, Books, Downloads, MCQ>
1 Introduction to the science of microbiology. Major divisions of microbial world and Relationships among them.
2 Different methods of classification of microbes and study of Bacteria, Fungi, Viruses, Rickettsiae, and Spirochetes.
3 Nutritional requirements, growth and cultivation of bacteria and virus. Study of different important media required for the growth of aerobic and anaerobic bacteria & fungi. Differential media, enriched media and selective media, maintenance of lab cultures.
4 Different methods used in the isolation and identification of bacteria with emphasis to different staining techniques and biochemical reactions. Counting of bacteria Total and Viable counting techniques.
5 Detailed study of different methods of sterilization including their merits and demerits. Sterilization methods for all pharmaceutical products. Detailed study of sterility testing of different pharmaceutical preparations. Brief information on Validation.
6 Disinfectants- Study of disinfectants, antiseptics, fungicidal, and virucidal agents factors affecting their activation and mechanism of action. Evaluation of bactericidal, bacteriostatic, and virucidal activities, evaluation of preservatives in pharmaceutical preparations.
7 Immunology- Immunity, Definition, Classification, General principles of natural immunity, Phagocytosis, acquired immunity( active and passive ). Antigens, chemical nature of antigens structure and formation of Antibodies, Antigen-Antibody reactions. Bacterial exotoxins and endotoxins. Significance of toxoids in active immunity, Immunization program, and importance of booster dose.
8 Diagnostic tests: Schick’s Test, Elisa test, Western Blot test, Southern Blot PCR Widal, QBC, Mantaux Peripheral smear. Study of malarial parasite.
9 Microbial culture sensitivity Testing: Interpretation of results Principles and methods of different microbiological assays, a microbiological assay of Penicillin, Streptomycin and vitamin B2 and B12. Standardisation of vaccines and sera.
10 Study of infectious diseases: Typhoid, Tuberculosis, Malaria, Cholera, Hepatitis, Meningitis, Syphilis & Gonorrhea and HIV.
Pharmaceutical microbiology Practical
1 Study of apparatus used in experimental microbiology
2 Sterilisation of glasswares. Preparation of media and sterilization.
3 Staining techniques – Simple staining; Gram’s staining; Negative staining
4 Study of motility characters
5 Enumeration of micro-organisms (Total and Viable)
6 Study of the methods of isolation of pure culture.
7 Biochemical testing for the identification of micro-organisms.
8 Cultural sensitivity testing for some micro-organisms.
9 Sterility testing for powders and liquids.
10 Determination of minimum inhibitory concentration.
11 Microbiological assay of antibiotics by cup plate method.
12 Microbiological assay of vitamins by Turbidometric method
13 Determination of RWC.
14 Diagnostic tests for some common diseases, Widal, malarial parasite.
Second Year Pharm D Subjects
|2.3||Pharmacognosy & Phytopharmaceuticals|
- Pharmaceutical Analysis I Theory
- Pharmaceutics I Theory
- Social Pharmacy Theory
- b pharmacy syllabus
- BIOSTATISTICS AND RESEARCH METHODOLOGY Theory
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