## Determination of particle size, and particle size distribution using the Microscopic method

**BP407P Physical Pharmaceutics II Practical**

STANDARD STAGE MICROMETER:

ïƒ¼ Standard stage micrometre is used to calibration of eye piece micrometre. Eye piece micrometre is a glass slide (7.5cm into 2.5cm) which has the scales engraved in the scale usefully 0.1mm is length. 1mm divided into 100 divisions. Thus smallest division least count of the stage micrometre represents 0.01mm or 10Âµm length.

ïƒ¼ In this experiment in the optical combination of 10x eye piece and 45x objective is used

ïƒ¼ The stage micrometre is least on the stage of the microscope. The objective is position to the centre of objective

ïƒ¼ Initially disc focus low power the scale of stage micro meter observed (100 divisions)

ïƒ¼ Now the objective is focus to high power (45x)

ïƒ¼ Two points were selected one point on the left side where divisions both scales coincide and another point on the right side

ïƒ¼ The number of small division that is eye piece were counted and big division stage micrometre were counted and recorded

1 eye piece = y/x Ã— 0.01mm 1 eye piece = y/x Ã— 10 Âµm

PROCEDURE:

Counting of the sample

- A small portion of given sample transfer to a clean slide
- One (or) two drops of liquid paraffin is added to the slide
- The sample is dispersed uniformly with help of brush and particles should be in depended and distribution should be uniform
- The cover slip is placed carefully entrancement of air bubbles is avoided
- The slide is placed the stage of microscope

Measurement of particle size:

â€¢ The slide is focus in low power (10x) the presence of individual particle is absorb (if aggregation or lumps are present the sample should be mounted again)

â€¢ The size of the each particles measure is terms of eye piece division

â€¢ A total 300 particles should be considered for size distribution analysis. Ideally 625 particles measure according to BPC.

REPORT:

From the graph it was found the particles were distributed uniformly from size range of 0-400Âµm.

according to BPC.

REPORT:

From the graph it was found the particles were distributed uniformly from size range of 0-400Âµm.

From the graph it was found the particles were distributed uniformly from size range of 0-400Âµm.

Physical Pharmaceutics II Practical

- Determination of particle size, and particle size distribution using the sieving method
- Determination of particle size, and particle size distribution using the Microscopic method
- Determination of bulk density, true density and porosity
- Determine the angle of repose and the influence of lubricant on the angle of repose
- Determination of viscosity of liquid using Ostwaldâ€™s viscometer
- Determination of sedimentation volume with the effect of different suspending agent
- Determination of sedimentation volume with the effect of different concentrations of single suspending agent
- Determination of viscosity of semisolid by using Brookfield viscometer
- Determination of reaction rate constant first order.
- Determination of reaction rate constant second order
- Accelerated stability studies

**Second Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise**