May 20, 2024

1.3 Medicinal Biochemistry Pharm D Syllabus, Notes, PDF, Books, Downloads

1.3 Medicinal Biochemistry Pharm D Syllabus, Notes, PDF, Books, Downloads

Medicinal Biochemistry Theory

1 Introduction to biochemistry

Cell and its biochemical organization,
Transport process across the cell membranes.
Energy-rich compounds; ATP, Cyclic AMP, and their biological significance.

2 Enzymes:

Definition; Nomenclature;
IUB classification;
Factor affecting enzyme activity; Enzyme action; enzyme inhibition.
Isoenzymes and their therapeutic and diagnostic applications;
Coenzymes and their biochemical role and deficiency diseases.

3 Carbohydrate metabolism:

Citric acid cycle (TCA cycle),
HMP shunt,
Metabolic disorders of carbohydrate metabolism (diabetes mellitus and glycogen storage diseases);
Glucose, Galactose tolerance test, and their significance;
Hormonal regulation of carbohydrate metabolism.

4 Lipid metabolism:

Oxidation of saturated (-oxidation); Ketogenesis and ketolysis; biosynthesis of fatty acids, and lipids; metabolism of cholesterol; Hormonal regulation of lipid metabolism. Defective metabolism of lipids (Atherosclerosis, fatty liver, hypercholesterolemia).

5 Biological oxidation:

Coenzyme system involved in Biological oxidation. Electron transport chain (its mechanism in energy capture; regulation and inhibition); Uncouplers of ETC; Oxidative phosphorylation;

6 Protein and amino acid metabolism:

protein turnover; nitrogen balance; Catabolism of Amino acids (Transamination, deamination & decarboxylation). Urea cycle and its metabolic disorders; production of bile pigments; hyperbilirubinemia, porphoria, jaundice. Metabolic disorder of Amino acids.

7 Nucleic acid metabolism:

Metabolism of purine and pyrimidine nucleotides; Protein synthesis; Genetic code; inhibition of protein synthesis; mutation and repair mechanism; DNA replication (semiconservative /onion peel models) and DNA repair mechanism.

8 Introduction to clinical chemistry:

Cell; composition; malfunction; Roll of the clinical chemistry laboratory.

9 The kidney function tests:

Role of the kidney; Laboratory tests for a normal function include-
a) Urine analysis (macroscopic and physical examination, quantitative and semiquantitative tests.)
b) Test for NPN constituents. (Creatinine /urea clearance, determination of blood and urine creatinine, urea, and uric acid)
c) Urine concentration test
d) Urinary tract calculi. (stones)

10 Liver function tests:

Physiological role of liver, metabolic, storage, excretory, protective, circulatory functions and function in blood coagulation.
a) Test for hepatic dysfunction-Bile pigments metabolism.
b) Test for hepatic function test- Serum bilirubin, urine bilirubin, and urine urobilinogen.
c) Dye tests of excretory function.
d) Tests based upon abnormalities of serum proteins. Selected enzyme tests.

11 Lipid profile tests:

Lipoproteins, composition, functions. Determination of serum lipids, total cholesterol, HDL cholesterol, LDL cholesterol, and triglycerides.

12 Immunochemical techniques

Immunochemical techniques for determination of hormone levels and protein levels in serum for endocrine diseases and infectious diseases. Radio Immunoassay (RIA) and Enzyme Linked Immuno Sorbent Assay (ELISA)

13 Electrolytes:

Body water, compartments, water balance, and electrolyte distribution. Determination of sodium, calcium potassium, chlorides, and bicarbonates in the body fluids.

Medicinal Biochemistry Practical

1 Qualitative analysis of normal constituents of urine
2 Qualitative analysis of abnormal constituents of urine
3 Quantitative estimation of urine sugar by Benedict’s reagent method
4 Quantitative estimation of urine chlorides by Volhard’s method
5 Quantitative estimation of urine creatinine by Jaffe’s method
6 Quantitative estimation of urine calcium by precipitation method
7 Quantitative estimation of serum cholesterol by Libermann Burchard’s method
8 Preparation of Folin Wu filtrate from blood
9 Quantitative estimation of blood creatinine
10 Quantitative estimation of blood sugar Folin-Wu tube method
11 Estimation of SGOT in serum
12 Estimation of SGPT in serum
13 Estimation of Urea in Serum
14 Estimation of Proteins in Serum
15 Determination of serum bilirubin
16 Determination of Glucose by means of Glucose oxidase
17 Enzymatic hydrolysis of Glycogen/Starch by Amylases
18 Study of factors affecting Enzyme activity. (pH & Temp.)
19 Preparation of standard buffer solutions and its pH measurements (any two)
20 Experiment on lipid profile tests
21 Determination of sodium, calcium and potassium in serum

First Year Pharm D Subjects Syllabus, Notes, PDF Books, MCQ

1.1Human Anatomy and Physiology
1.3Medicinal  Biochemistry
1.4Pharmaceutical Organic Chemistry
1.5Pharmaceutical Inorganic Chemistry
1.6Remedial Mathematics/ Biology

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