Interferon Interleukin production rDNA technology notes
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- Recombinant human interleukin is known as antiviral proteins (interferon).
- Interferon is a protein produced by human cells in response to an antigen.
- The human cells respond to the antigen by producing antibodies which are proteins made up of 165 amino acids.
- In immuno-compromised patients, these antibodies cannot be produced so vaccination is required.
- Interferon is a non-glycosylated protein.
- It does not require post-translational modification.
- The production is done by affinity chromatography.
- Murine monoclonal antibodies (protein) specific to the interferon are used
- The interferon gene is isolated from human DNA.
- It is placed in the plasmid pUC13.
- Then plasmid is placed into the host i.e. E.coli.
- Murine monoclonal antibody is fixed in glass column.
- The E.coli culture containing the interferon, after lysis of cell wall is poured into the column.
- The interferon binds to murine monoclonal antibody and in this way other component of E.coli will be separated from interferon.
- The interferon molecules are washed from the column by using buffers and suitable formulation is prepared.
SIMPLIFIED CONCEPTS OF BIOTECHNOLOGY By, Dr. Pramod Kadu & Ms. Suchita Vishwakarma
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