Interferon Interleukin Production rDNA: How It Works

Interferon Interleukin production rDNA technology notes

BP605T Pharmaceutical Biotechnology Theory Books, PDF Notes, Download

Recombinant human interleukin is known as antiviral proteins (interferon).
Interferon is a protein produced by human cells in response to an antigen.
The human cells respond to the antigen by producing antibodies which are proteins made up of 165 amino acids.
In immuno-compromised patients, these antibodies cannot be produced so vaccination is required.
Interferon is a non-glycosylated protein.
It does not require post-translational modification.
The production is done by affinity chromatography.
Murine monoclonal antibodies (protein) specific to the interferon are used

Murine monoclonal antibody is fixed in glass column.
The E.coli culture containing the interferon, after lysis of cell wall is poured into the column.
The interferon binds to murine monoclonal antibody and in this way other component of E.coli will be separated from interferon.
The interferon molecules are washed from the column by using buffers and suitable formulation is prepared.

Reference: SIMPLIFIED CONCEPTS OF BIOTECHNOLOGY By, Dr. Pramod Kadu & Ms. Suchita Vishwakarma

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T Y B Pharm Sem V	T Y B Pharm Sem VI
BP501T Medicinal Chemistry II Theory	BP601T Medicinal Chemistry III Theory
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BP503T Pharmacology II Theory	BP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II Theory	BP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence Theory	BP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I Practical	BP606T Quality Assurance Theory
BP507P Pharmacology II Practical	BP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II Practical	BP608P Pharmacology III Practical
	BP609P Herbal Drug Technology Practical