May 30, 2024

Identification Test for Albumin: Biochemistry Practical, Lab Manual

IdentificationTest for Albumin: Biochemistry Practical, Lab Manual

BP209P Biochemistry Practical / B Pharmacy Syllabus and Notes


S.NO.EXPERIMENTOBSERVATIONINFERENCE
1XANTHOPROTEIN TEST: To 3ml of protein solution add 1ml conc. nitric acid boil for about half a minute. Cool and observe the yellow colour. Now, add 2ml of conc. ammonia or 40% sodium hydroxide and observe the change in colour after the addition of alkali.yellow colour is appearsPresence of aromatic amino acid.
2MILLON’S TEST: To 1ml of protein solution add 1ml of 10% mercuric nitrate (prepared in 10% sulphuric acid). Boil gently for half a minute. Cool and add 3 drops of 1% solution of sodium nitrate. Mix and observe.The          red          colour precipitate is obtainedPresence of tyrosine in a solution
3SULPHUR TEST: To 3ml of protein solution add an equal volume of 40% sodium hydroxide and boil for 3 minutes. Cool and then add 1ml of lead acetate solution.No dark grey or black precipitate is obtained.Absence of sulphur-containing amino acid cysteine and cystine. Methionine does not answer this test due to the presence of thioether linkage which does not allow the release of sulphur in this reaction.
4HELLER’S TEST: Take 3ml of con. nitric acid and add 2ml of urine along the sides of the test tube.A white ring of meta proteins appears at the junction of the fluids.Presence of proteins like albumin & globulins.
5PRECIPITATION BY NEUTRAL SALT: HALF SATURATION: Take 3ml of protein solution in a test tube; add an equal volume of saturated ammonium sulphate solution to it, mixed and allowed to stand. FULL SATURATION: Take 3ml of protein solution, add ammonium sulphate salt to it and keep on adding and at the same time mix till protein solution become saturated.No white precipitate is formed.               White precipitate is formed.Absence of     Globulin, Casein.                 Presence of Albumin

REPORT:

The given sample was found to be Albumin.


First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

F Y B Pharm Sem-IF Y B Pharm Sem-II
BP101T Human Anatomy and Physiology I TheoryBP201T Human Anatomy and Physiology II – Theory
BP102T Pharmaceutical Analysis I TheoryBP202T Pharmaceutical Organic Chemistry I Theory
BP103T Pharmaceutics I TheoryBP203T Biochemistry – Theory
BP104T Pharmaceutical Inorganic Chemistry TheoryBP204T Pathophysiology – Theory
BP105T Communication skills TheoryBP205T Computer Applications in Pharmacy Theory
BP106RBT Remedial BiologyBP206T Environmental sciences – Theory
BP106RMT Remedial Mathematics TheoryBP207P Human Anatomy and Physiology II Practical
BP107P Human Anatomy and Physiology PracticalBP208P Pharmaceutical Organic Chemistry I Practical
BP108P Pharmaceutical Analysis I PracticalBP209P Biochemistry Practical
BP109P Pharmaceutics I PracticalBP210P Computer Applications in Pharmacy Practical
BP110P Pharmaceutical Inorganic Chemistry Practical
BP111P Communication skills Practical
BP112RBP Remedial Biology Practical

Suggested readings:


BP209P Biochemistry Practical, Lab Manual, PDF, Journal, Book, D Pharm B Pharmacy Syllabus and Notes