Honey: Physical and chemical tests / Analysis of crude drugs
Chemical test for Honey
|1.||Fiehe’s test Take about 3ml of honey + 2ml of ether and shake thoroughly and allow the 2 layers to separate and evaporate to dryness. The upper etherial layer is separated and put in a china dish and evaporate, to the residue add 1% resorcinol and HCl.||Transient pink colour Permanent red colour||Pur honey Adulterated honey(Invert sugar)|
|2.||Molisch’s Test Honey is treated with alpha Napthol and concentrated sulphuric acid||Purple colour||Presence carbohydrate|
|3.||Reducing Sugar Test Heat honey to this add a drop of mixture of Fehling’s solution A & B||Brick red colour of cuprous oxide||Presence monosaccharide|
From the above morphological characteristics and chemical tests, the crude drug is identified as Honey.
Kokate CK. Practical Pharmacognosy, 4 edition, VallabhPrakashan. Delhi; 1994: 98
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