February 22, 2024

Estimation of calcium gluconate by Complexometric titration

Estimation of calcium gluconate by Complexometric titration

Calcium gluconate is a calcium salt of gluconic acid and is commonly used as a calcium supplement. Complexometric titration can be used to determine the amount of calcium present in a calcium gluconate sample.

In complexometric titration, a chelating agent, such as ethylenediaminetetraacetic acid (EDTA), is used to form a complex with the calcium ions in the sample solution. The addition of a metal ion indicator, such as Eriochrome Black T, helps to indicate the endpoint of the titration.

To carry out the titration, a known quantity of the calcium gluconate sample is dissolved in water, and the pH is adjusted to around 10 with the addition of ammonium hydroxide. Then, a small amount of Eriochrome Black T indicator solution is added to the solution, and the titration is initiated by adding the EDTA solution.

As the EDTA solution is slowly added, it forms a complex with the calcium ions, and the color of the solution changes from red to blue. The endpoint of the titration is reached when all the calcium ions have reacted with the EDTA, and the solution turns from blue to pink.

The amount of EDTA solution added to the sample can be used to calculate the amount of calcium present in the sample, using stoichiometry of the reaction.

It is important to ensure that the calcium gluconate sample is completely dissolved in water and free from any other impurities that may affect the accuracy of the titration. Additionally, the EDTA solution should be standardized against a known quantity of calcium before use to ensure accurate results.

Overall, complexometric titration can be a reliable and accurate method for the estimation of calcium gluconate, and can be used in the pharmaceutical industry and research laboratories.

First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

F Y B Pharm Sem-IF Y B Pharm Sem-II
BP101T Human Anatomy and Physiology I TheoryBP201T Human Anatomy and Physiology II – Theory
BP102T Pharmaceutical Analysis I TheoryBP202T Pharmaceutical Organic Chemistry I Theory
BP103T Pharmaceutics I TheoryBP203T Biochemistry – Theory
BP104T Pharmaceutical Inorganic Chemistry TheoryBP204T Pathophysiology – Theory
BP105T Communication skills TheoryBP205T Computer Applications in Pharmacy Theory
BP106RBT Remedial BiologyBP206T Environmental sciences – Theory
BP106RMT Remedial Mathematics TheoryBP207P Human Anatomy and Physiology II Practical
BP107P Human Anatomy and Physiology PracticalBP208P Pharmaceutical Organic Chemistry I Practical
BP108P Pharmaceutical Analysis I PracticalBP209P Biochemistry Practical
BP109P Pharmaceutics I PracticalBP210P Computer Applications in Pharmacy Practical
BP110P Pharmaceutical Inorganic Chemistry Practical
BP111P Communication skills Practical
BP112RBP Remedial Biology Practical

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