DETERMINATION OF TIDAL VOLUME BP112RBP REMEDIAL BIOLOGY Practical
Aim:
To measure the tidal volume.
Requirements:
Spirometer, Kymograph, Disinfectant, Eye protection, Soda lime
Theory and Conclusion:
The tidal volume is the volume of air breathed in and out in one breath at rest. The tidal volume for most adults is only about 0.5 dm3. It can be measured with the help of a spirometer.
The general principle behind a spirometer is simple. It is effectively a tank of water with an air-filled chamber suspended in the water. t is set up so that adding air to the chamber makes the lid of the chamber rise in the water, and removing air makes it fall. Movements of the chamber are recorded using a kymograph (pen writing on a rotating drum). Tubes run from the chamber to a mouthpiece and back again. Breathing in and out through the tubes makes the lid of the chamber fall and rise. The volume of air the person inhales and exhales can be calculated from the distance the lid moves.
Procedure:
1. Insert a canister containing soda lime between the mouthpiece and the floating chamber. This absorbs the carbon dioxide that the subject exhales.
2. Fill the spirometer with oxygen. A disinfected mouthpiece is attached to the tube.
3. Put a nose clip on the subject to be tested, and place the mouthpiece in the mouth.
4. Switch ON the recording apparatus and at the end of an exhaled breath turn the tap so that the mouthpiece is connected to the spirometer chamber. The trace will move down as the person breathes in.
5. After breathing normally, the subject should take as deep a breath as possible and then exhale as much air as possible before returning to normal breathing.
Below is the standard trace showing how to read off the residual volume, expiratory reserve volume, tidal volume, inspiratory reserve volume, vital capacity and total lung capacity.
Remedial Biology Practicals
- Introduction to experiments in biology a) Study of Microscope b) Section cutting techniques c) Mounting and staining d) Permanent slide preparation 2. Study of cell and its inclusions 3. Study of Stem, Root, Leaf, seed, fruit, flower and their modifications 4. Detailed study of frog by using computer models 5. Microscopic study and identification of tissues pertinent to Stem, Root Leaf, seed, fruit and flower 6. Identification of bones 7. Determination of blood group 8. Determination of blood pressure 9. Determination of tidal volume
First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise
First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise