September 30, 2023

Determination of Bleeding Time

Determination of Bleeding Time

Determination of Bleeding Time

BP107P Human Anatomy and Physiology Practical


To determine the bleeding time of a patient.


The time required for the complete stopping of blood flow from the punctured blood vessels is called the bleeding time. Normally it is 1-3 minutes for a normal human’s blood. Normal clotting time and bleeding time values differ because bleeding time is the time for stopping bleeding by the formation of fibrin network on the surface of punctured skin; that is it is the surface phenomenon. But the clotting time is the time for clotting the whole blood, collected in the capillary tube; therefore it is a volume phenomenon. For this reason, clotting time is more than the bleeding time when determined by conventional methods.

Clinical significance

It plays significant role
i) to study hemorrhagic disorders.
ii) to study the coagulation defects
iii) to have an idea about the platelet count of the patient. Bleeding time is prolonged in a few disorders like vascular lesions, platelet defect, severe liver disease, uremia, and anti-coagulant drug administration.


Sterilized needle, filter paper, cotton, spirit, and stopwatch.

Procedure (Duke’s method)

The finger of a subject is sterilized with spirit and pricked with a sterilized needle. Time of pricking is noted. Take the stain of the punctured point on a filter paper for 30 seconds and keep taking the stain of blood in 20-second intervals until the bleeding stops. The time of no stain has come is noted properly; it is the bleeding time of the subject.


Following precautions should be enforced
i) Needle should be sterilized.
ii) A fain stain of blood should not be avoided.
iii) Time should be noted properly.

Human Anatomy and Physiology Practical Syllabus

  1. Study of a compound microscope.
  2. Microscopic study of epithelial and connective tissue
  3. Microscopic study of muscular and nervous tissue
  4. Identification of axial bones
  5. Identification of appendicular bones
  6. Introduction to hemocytometry.
  7. Enumeration of white blood cell (WBC) count
  8. Enumeration of total red blood corpuscles (RBC) count
  9. Determination of the bleeding time
  10. Determination of clotting time
  11. Estimation of haemoglobin content
  12. Determination of blood group.
  13. Determination of erythrocyte sedimentation rate (ESR).
  14. Determination of heart rate and pulse rate.
  15. Recording of blood pressure.

First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

F Y B Pharm Sem-IS Y B Pharm Sem-II
BP101T Human Anatomy and Physiology I TheoryBP201T Human Anatomy and Physiology II – Theory
BP102T Pharmaceutical Analysis I TheoryBP202T Pharmaceutical Organic Chemistry I Theory
BP103T Pharmaceutics I TheoryBP203T Biochemistry – Theory
BP104T Pharmaceutical Inorganic Chemistry TheoryBP204T Pathophysiology – Theory
BP105T Communication skills TheoryBP205T Computer Applications in Pharmacy Theory
BP106RBT Remedial BiologyBP206T Environmental sciences – Theory
BP106RMT Remedial Mathematics TheoryBP207P Human Anatomy and Physiology II Practical
BP107P Human Anatomy and Physiology PracticalBP208P Pharmaceutical Organic Chemistry I Practical
BP108P Pharmaceutical Analysis I PracticalBP209P Biochemistry Practical
BP109P Pharmaceutics I PracticalBP210P Computer Applications in Pharmacy Practical
BP110P Pharmaceutical Inorganic Chemistry Practical
BP111P Communication skills Practical
BP112RBP Remedial Biology Practical

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