February 18, 2025

701T Instrumental Methods of Analysis Theory, Notes, PDF, Books

BP701T Instrumental Methods of Analysis Theory, Notes, PDF, Books


UNIT I UV Visible spectroscopy, Fluorimetry

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation, and deviations.
Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.
Applications – Spectrophotometric titrations, Single-component and multi-component analysis

Fluorimetry

Theory, Concepts of singlet, doublet, and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation, and applications


UNIT II Spectroscopy

IR spectroscopy

Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations
Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry

Principle, interferences, instrumentation, and applications

Atomic absorption spectroscopy

Principle, interferences, instrumentation, and applications

Nepheloturbidometry

Principle, instrumentation, and applications


UNIT III Introduction to chromatography

Introduction to chromatography

Adsorption and partition column chromatography

Methodology, advantages, disadvantages, and applications.

Thin-layer chromatography

Introduction, Principle, Methodology, Rf values, advantages, disadvantages, and applications.

Paper chromatography

Introduction, methodology, development techniques, advantages, disadvantages, and applications

Electrophoresis

Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications


UNIT IV Gas chromatography, HPLC

Gas chromatography

Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages, and applications

High-performance liquid chromatography (HPLC)

Introduction, theory, instrumentation, advantages, and applications.


UNIT V Ion exchange, Gel, Affinity Chromatography

Ion exchange chromatography

Introduction, classification, ion exchange resins, properties, mechanism of the ion exchange process, factors affecting ion exchange, methodology, and applications

Gel chromatography

Introduction, theory, instrumentation, and applications

Affinity chromatography

Introduction, theory, instrumentation, and applications


  1. Instrumental Methods of Chemical Analysis by B.K Sharma
  2. Organic spectroscopy by Y.R Sharma
  3. Text book of Pharmaceutical Analysis by Kenneth A. Connors
  4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
  5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
  6. Organic Chemistry by I. L. Finar
  7. Organic spectroscopy by William Kemp
  8. Quantitative Analysis of Drugs by D. C. Garrett
  9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
  10. Spectrophotometric identification of Organic Compounds by Silverstein

Final Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

Final Year B Pharm Sem VIIBP701T Instrumental Methods of Analysis Theory
BP702T Industrial Pharmacy TheoryBP703T Pharmacy Practice Theory
BP704T Novel Drug Delivery System TheoryBP705 P Instrumental Methods of Analysis Practical
Final Year B Pharm Sem VIIBP801T Biostatistics and Research Methodology Theory
BP802T Social and Preventive Pharmacy TheoryBP803ET Pharmaceutical Marketing Theory
BP804ET Pharmaceutical Regulatory Science TheoryBP805ET Pharmacovigilance Theory
BP806ET Quality Control and Standardization of Herbals TheoryBP807ET Computer-Aided Drug Design Theory
BP808ET Cell and Molecular Biology TheoryBP809ET Cosmetic Science Theory
BP810ET Experimental Pharmacology TheoryBP811ET Advanced Instrumentation Techniques Theory
BP812ET Dietary supplements and NutraceuticalsPharmaceutical Product Development

Suggested readings


Recommended readings: