BP701T Instrumental Methods of Analysis Theory, Notes, PDF, Books
Table of contents
UNIT I UV Visible spectroscopy, Fluorimetry
UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation, and deviations.
Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.
Applications – Spectrophotometric titrations, Single-component and multi-component analysis
Fluorimetry
Theory, Concepts of singlet, doublet, and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation, and applications
UNIT II Spectroscopy
IR spectroscopy
Introduction, fundamental modes of vibrations in polyatomic molecules, sample handling, factors affecting vibrations
Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications
Flame Photometry
Principle, interferences, instrumentation, and applications
Atomic absorption spectroscopy
Principle, interferences, instrumentation, and applications
Nepheloturbidometry
Principle, instrumentation, and applications
UNIT III Introduction to chromatography
Introduction to chromatography
Adsorption and partition column chromatography
Methodology, advantages, disadvantages, and applications.
Thin-layer chromatography
Introduction, Principle, Methodology, Rf values, advantages, disadvantages, and applications.
Paper chromatography
Introduction, methodology, development techniques, advantages, disadvantages, and applications
Electrophoresis
Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications
UNIT IV Gas chromatography, HPLC
Gas chromatography
Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages, and applications
High-performance liquid chromatography (HPLC)
Introduction, theory, instrumentation, advantages, and applications.
UNIT V Ion exchange, Gel, Affinity Chromatography
Ion exchange chromatography
Introduction, classification, ion exchange resins, properties, mechanism of the ion exchange process, factors affecting ion exchange, methodology, and applications
Gel chromatography
Introduction, theory, instrumentation, and applications
Affinity chromatography
Introduction, theory, instrumentation, and applications
Recommended Books (Latest Editions)
- Instrumental Methods of Chemical Analysis by B.K Sharma
- Organic spectroscopy by Y.R Sharma
- Text book of Pharmaceutical Analysis by Kenneth A. Connors
- Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
- Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
- Organic Chemistry by I. L. Finar
- Organic spectroscopy by William Kemp
- Quantitative Analysis of Drugs by D. C. Garrett
- Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
- Spectrophotometric identification of Organic Compounds by Silverstein
Final Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise
Suggested readings
Recommended readings:
- Computer Applications in Pharmacy Practical
- b pharmacy syllabus
- Pharmaceutics I Practical
- Pharmaceutical Biotechnology Theory
- Pathophysiology