February 19, 2025

Assay of Ferrous sulfate IP Pharmaceutical Analysis I Practical

conductivity cell

Assay of Ferrous sulphate IP Pharmaceutical Analysis I Practical


Pharmaceutical Analysis I Practical: Limit test for (1) Chloride (2) Sulphate (3) Iron (4) Arsenic Preparation and standardisation of (1) Sodium hydroxide (2) Sulphuric acid (3) Sodium thiosulfate (4) Potassium permanganate (5) Ceric ammonium sulphate Assay (1) Ammonium chloride (2) Ferrous sulphate(3) Copper sulphate (4) Calcium gluconate (5) Hydrogen peroxide (6) Sodium benzoate (7) Sodium Chloride Determination of Normality (1) Conductometric titration of strong acid against a strong base (2) Conductometric titration of strong acid and weak acid against a strong base (3) Potentiometric titration


Aim

To determine the percent purity of Ferrous sulfate in a given sample

Principle

This determination is cerimetric type of redox titration. Ceric ammonium nitrate is a strong oxidizing agent that oxidizes ferrous ions to ferric ions and undergoes reduction to convert from ceric ion to cerous ion. Ferroin sulfate is used as an indicator which is a red-coloured complex of ferrous ions with 1, 10- phenanthroline. At the equivalence point excess of ceric ions converts ferrous in ferroin to ferric which changes the colour of the solution from red to light green colour

Standard

Ferrous sulphate contains not less than 98% and not more than 105 % of FeSO4.7H2O

Procedure

Dissolve 2.5g of sodium bicarbonate in a mixture of 150 ml of water and 10 ml of sulphuric acid. When effervescence ceases, add about 0.5 g of substance under examination, accurately weighed, shake gently to dissolve, and titrate with 0.1 M Ceric ammonium nitrate using 0.1 ml of ferroin solution as an indicator until the red colour disappears

Reference

Indian Pharmacopoeia 2014; Vol. II; Pg. No. 1753-1754.


Synopsis and Viva questions

What is Ferrous Sulfate IP?

Ferrous Sulfate IP is a pharmaceutical compound widely used for its iron supplement properties. In pharmaceutical analysis, it’s a crucial component in various formulations due to its stability and effectiveness.

Why is the assay of Ferrous Sulfate IP essential?

The assay determines the purity and concentration of Ferrous Sulfate IP in a pharmaceutical sample. It ensures compliance with regulatory standards and verifies the efficacy of the product.

What methods are used to assay Ferrous Sulfate IP?

The assay can be performed through various methods like titrimetric, spectrophotometric, or gravimetric methods. Titrimetrically, it’s often assessed using standard solutions like potassium permanganate or cerium sulfate.

What challenges might arise during the assay process?

Challenges could include interference from impurities, oxidation of ferrous to ferric iron, or difficulties in achieving reproducibility due to varying sample characteristics.

How should one prepare for the assay of Ferrous Sulfate IP?

Preparation involves ensuring proper calibration of instruments, accurate measurement of reagents, and careful sample handling to avoid contamination.

What precautions should be taken during the assay?

Avoid exposure to air to prevent oxidation, maintain consistent temperature and pH conditions, and adhere strictly to the specified procedures outlined in the pharmacopeia.

What does the assay result indicate?

The assay result signifies the percentage of the active ingredient (Ferrous Sulfate IP) present in the sample, validating its potency and quality.

How do variations in assay results impact pharmaceutical formulations?

Variations can affect dosage accuracy and therapeutic efficacy, potentially leading to inadequate treatment outcomes or unexpected side effects.

Why is it crucial to adhere to the assay protocol for Ferrous Sulfate IP?

Adherence to the assay protocol ensures the reliability and consistency of the pharmaceutical product, safeguarding its quality and effectiveness for patients.

First Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

F Y B Pharm Sem-IF Y B Pharm Sem-II
BP101T Human Anatomy and Physiology I TheoryBP201T Human Anatomy and Physiology II – Theory
BP102T Pharmaceutical Analysis I TheoryBP202T Pharmaceutical Organic Chemistry I Theory
BP103T Pharmaceutics I TheoryBP203T Biochemistry – Theory
BP104T Pharmaceutical Inorganic Chemistry TheoryBP204T Pathophysiology – Theory
BP105T Communication skills TheoryBP205T Computer Applications in Pharmacy Theory
BP106RBT Remedial BiologyBP206T Environmental sciences – Theory
BP106RMT Remedial Mathematics TheoryBP207P Human Anatomy and Physiology II Practical
BP107P Human Anatomy and Physiology PracticalBP208P Pharmaceutical Organic Chemistry I Practical
BP108P Pharmaceutical Analysis I PracticalBP209P Biochemistry Practical
BP109P Pharmaceutics I PracticalBP210P Computer Applications in Pharmacy Practical
BP110P Pharmaceutical Inorganic Chemistry Practical
BP111P Communication skills Practical
BP112RBP Remedial Biology Practical