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Assay of Atropine: Medicinal Chemistry Practical

BP406P Medicinal Chemistry I Practical


AIM:

To carry out the assay of atropine.

REFERENCE:

  1. Indian Pharmacopoeia 2018 page no:1600-01.
  • A textbook of Medicinal Chemistry-I,Pragi Arora,Varun Arora,Davinder Kumar,Page no:281,282

REQUIREMENTS:

Perchloric acid (0.1M),atropine,glacial acetic acid,crystal violet solution (0.2%w/v in acetic acid),acetone,methyl orange indicator, conical flask, burette, beaker.

PRINCIPLE:

Atropine is assayed by non-aqueous titration which is generally used for the titration of weak acid with weak base. In this titration non-aqueous solvent perchloric acid is used and crystal violet is used as an indicator. At the end point blue colour is obtained.

PROCEDURE:

  1. STANDARDISATION OF PERCHLORIC ACID (0.1N)

Dissolved  0.5g of potassium hydrogen phthalate in 25ml of glacial acetic acid and few drops of 5%w/v crystal violet indicator. Titrated the solution with 0.1N perchloric acid till blue green colour appears.

ASSAY OF ATROPINE

Weighed accurately 400mg of atropine and dissolved it in 50ml of glacial acetic acid and added a drop of crystal violet indicator. Titrated this solution with 0.1N perchloric acid until green color is obtained end point.


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BP301T Pharmaceutical Organic Chemistry II TheoryBP401T Pharmaceutical Organic Chemistry III Theory
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BP304T Pharmaceutical Engineering TheoryBP404T Pharmacology I Theory
BP305P Pharmaceutical Organic Chemistry II Practical BP405T Pharmacognosy I Theory
BP306P Physical Pharmaceutics I PracticalBP406P Medicinal Chemistry I Practical
BP307P Pharmaceutical Microbiology PracticalBP407P Physical Pharmaceutics II Practical
BP308P Pharmaceutical Engineering PracticalBP408P Pharmacology I Practical
BP409P Pharmacognosy I Practical

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