Assay of Isonicotinic acid hydrazide
BP607P Medicinal Chemistry III Practical : I Preparation of drugs Sulphanilamide * 7-Hydroxy, 4-methyl coumarin * Chlorobutanol * Triphenyl imidazole * Tolbutamide * Hexamine II Assay of drugs Isonicotinic acid hydrazide * Chloroquine * Metronidazole * Dapsone * Chlorpheniramine maleate * Benzyl penicillin III Microwave irradiation technique Synthesis of Phenytoin by Microwave * Synthesis of Aspirin by Microwave IV Drawing structures and reactions using chem draw®
Aim:
To determine the percentage purity of given sample of Isonicotic acid hydrazide tablet.
Principle:
Isonicotinic acid hydrazide/ isoniazid is an anti-tubercular drug. It is assayed by the direct titration of Potassium bromate with addition of potassium bromide in the presence of acid medium using hydrochloric acid.
During oxidation reaction liberated bromine reacts with isoniazid in aqueous solution to form isonicotinic acid. Azo dye methyl red indicator is used in this titration, decolorized the red colour is the end point.
Reaction:
Procedure:
Preparation of 0.0167 M Potassium bromate solution:
About 5.566gm of Potassium bromate was dissolved in water and made up to 1000 ml with distilled water.
Standardisation of 0.0167 M Potassium bromate:
20 ml of above solution was transferred in a glass stopper flask and 3 g of Potassium iodide and followed with 3 ml of HCl was added.
Allow to stand for 5 min, titrate liberated Iodine with 0.1 M sodium thiosulphate adding 3 ml of starch solution TS and the end point is approached.
Concentration of 0.0167 M Potassium bromate= Molarity of Sodium thiosulphate / Volume of Potassium bromate
Assay of Isonicotinic acid hydrazide tablet
Twenty tablets were weighed accurately and pulverized.
A weighed quantity of the tablet power equivalent to 0.25 mg INH was transferred into a clean and dry 100 ml volumetric flask, then sufficient water was added to produce 100 ml. 20 ml of above solution was taken.
Then 100 ml of water, 20 ml of hydrochloric acid and 0.2 gm of Potassium bromide were added.
Then titrated against slowly with continuous shaking with 0.0167M potassium bromate using 0.05ml of methyl red as an indicator until red colour disappears.
Each ml of 0.0167M Potassium bromate KBrO3 = 0.003439g of C6H7N3O.
Report:
The Molarity of 0.1 M Sodium nitrite
The percentage purity of given isonicotinic acid Hydrazide tablet was found to be:
Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise