Determination of acute skin irritation test: Pharmacology Practical Manual

Aim:

To perform the acute skin irritation

Principle:

The test chemical to be tested is applied in a single dose to the skin of an experimental animal, untreated skin areas of the test animal serve as the control. The degree of irritation/ corrosion is read and scored at specified intervals. Animals showing continuing signs of serve distress and/or pain at any stage of the test should be humanely killed, and the test chemical assessed accordingly.

Procedure

The test chemical should be applied to a dorsal/ flank (approximately 6cm²) of skin and covered with a gauze patch. In cases in which direct application is not possible (eg: liquids or some pastes), the test chemical should first be applied to the gauze patch, which is then applied to the skin. The patch should be loosely held in contact with the skin by means of a suitable semi-occlusive dressing for the duration of the exposure period. If the test chemical is applied to the patch, it should be attached to the skin in such a manner that there is good contact & uniform distribution of the test chemical on the skin. In the liquid test, chemicals are generally used undiluted. When testing solids, the test chemical should be diluted with the smallest amount if water (or another suitable vehicle) is sufficient to ensure good skin contact. At the end of the exposure period, which is normally 4 hours, the residual test chemical should be removed.

Dose level:

A dose of 0.5ml of liquid or 0.5g of solid or paste is applied to the site.

Observation:

The degree of irritation/ corrosion is read and scored at specified intervals.

Third Year B Pharm Notes, Syllabus, Books, PDF Subjectwise/Topicwise

T Y B Pharm Sem V	T Y B Pharm Sem VI
BP501T Medicinal Chemistry II Theory	BP601T Medicinal Chemistry III Theory
BP502T Industrial Pharmacy Theory	BP602T Pharmacology III Theory
BP503T Pharmacology II Theory	BP603T Herbal Drug Technology Theory
BP504T Pharmacognosy II Theory	BP604T Biopharmaceutics and Pharmacokinetics Theory
BP505T Pharmaceutical Jurisprudence Theory	BP605T Pharmaceutical Biotechnology – Theory
BP506P Industrial Pharmacy I Practical	BP606T Quality Assurance Theory
BP507P Pharmacology II Practical	BP607P Medicinal chemistry III Practical
BP508P Pharmacognosy II Practical	BP608P Pharmacology III Practical
	BP609P Herbal Drug Technology Practical

Determination of acute skin irritation test: Pharmacology Practical Manual